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Autor:
Thomas-Benjamin Seiler, Markus Brinkmann, Leonie Nüßer, Beat Thalmann, Andreas Schiwy, Henner Hollert, Kathrin Eichbaum, Brigitte Thoms, Gabriele Guder, Ines Thiem, Kerstin Winkens, Sebastian Buchinger, Georg Reifferscheid
Publikováno v:
Nature Protocols. 10:1728-1741
This protocol describes a quantitative and robust 96-well-plate-reader-based assay for the measurement of ethoxyresorufin-O-deethylase (EROD) activity using the rat hepatoma cell line H4IIE. The assay can be used to determine the cytochrome P450 subf