Výsledky vyhledávání - "Gabriela Sobiewska"

Cloning, Expression, and Purification of the His6-Tagged Thermostable β-Galactosidase from Pyrococcus woesei in Escherichia coli and Some Properties of the Isolated Enzyme

Autor: Józef Kur, Gabriela Sobiewska, Sławomir Dąabrowski, Jozef Synowiecki, Jadwiga Maciuńska

Publikováno v: Protein Expression and Purification. 19:107-112

In the previous study we cloned Pyrococcus woesei gene coding thermostable beta-galactosidase into pET30-LIC expression plasmid. The nucleotide sequence revealed that beta-galactosidase of P. woesei consists of 510 amino acids and has a molecular wei

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::133b78f3db53f26ed62bcb184444baa4
https://doi.org/10.1006/prep.2000.1231

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Thermal profile with alternately raised and lowered annealing temperature improves the PCR amplification using highly degenerate primers

Autor: Paweł Sachadyn, Gabriela Sobiewska, Józef Kur

Publikováno v: Scopus-Elsevier

The proposed here PCR thermal profile improves the specificity and efficiency of PCR using highly degenerate primers, especially in the case of larger PCR products (around 2000 bp and more). The improvement is achieved by the use of a specific anneal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a4294e3034f064e6e0368862e76eded
https://pubmed.ncbi.nlm.nih.gov/9918495

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