Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Gabriel T. Filsinger"'
Autor:
Lindsey R. Robinson-McCarthy, Alexander J. Mijalis, Gabriel T. Filsinger, Helena de Puig, Nina M. Donghia, Thomas E. Schaus, Robert A. Rasmussen, Raphael Ferreira, Jeantine E. Lunshof, George Chao, Dmitry Ter-Ovanesyan, Oliver Dodd, Erkin Kuru, Adama M. Sesay, Joshua Rainbow, Andrew C. Pawlowski, Timothy M. Wannier, Nicolaas M. Angenent-Mari, Devora Najjar, Peng Yin, Donald E. Ingber, Jenny M. Tam, George M. Church
Publikováno v:
Microbiology Spectrum, Vol 9, Iss 2 (2021)
ABSTRACT The coronavirus disease 2019 (COVID-19) pandemic has brought about the unprecedented expansion of highly sensitive molecular diagnostics as a primary infection control strategy. At the same time, many laboratories have shifted focus to sever
Externí odkaz:
https://doaj.org/article/ea251481fef24f6ab2c6dae008778d96
Autor:
Gleb Kuznetsov, Daniel B. Goodman, Gabriel T. Filsinger, Matthieu Landon, Nadin Rohland, John Aach, Marc J. Lajoie, George M. Church
Publikováno v:
Genome Biology, Vol 18, Iss 1, Pp 1-12 (2017)
Abstract We present a method for identifying genomic modifications that optimize a complex phenotype through multiplex genome engineering and predictive modeling. We apply our method to identify six single nucleotide mutations that recover 59% of the
Externí odkaz:
https://doaj.org/article/6d97b73a33114393895f5b605eecfd99
Autor:
Erkin Kuru, George Chao, Donald E. Ingber, Gabriel T. Filsinger, Timothy M. Wannier, George M. Church, Alexander J. Mijalis, Nicolaas M. Angenent-Mari, Devora Najjar, Nina M. Donghia, Oliver Dodd, Raphael Ferreira, Joshua Rainbow, Lindsey R. Robinson-McCarthy, Thomas E. Schaus, Andrew C. Pawlowski, Peng Yin, Jenny M. Tam, Adama M. Sesay, Jeantine E. Lunshof, Helena de Puig, Robert A. Rasmussen, Dmitry Ter-Ovanesyan
Publikováno v:
Microbiology Spectrum
Microbiology Spectrum, Vol 9, Iss 2 (2021)
Microbiology Spectrum, 9(2):e00313-21. AMER SOC MICROBIOLOGY
Microbiology Spectrum, Vol 9, Iss 2 (2021)
Microbiology Spectrum, 9(2):e00313-21. AMER SOC MICROBIOLOGY
The coronavirus disease 2019 (COVID-19) pandemic has brought about the unprecedented expansion of highly sensitive molecular diagnostics as a primary infection control strategy. At the same time, many laboratories have shifted focus to severe acute r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f97bc8efe1c0b9f388e03cee3bd58358
https://research.rug.nl/en/publications/638d132c-04ac-4449-9dda-1b6cc311e8ec
https://research.rug.nl/en/publications/638d132c-04ac-4449-9dda-1b6cc311e8ec
Autor:
Brian J Caldwell, Ekaterina Zakharova, Gabriel T Filsinger, Timothy M Wannier, Jordan P Hempfling, Lee Chun-Der, Dehua Pei, George M Church, Charles E Bell
Publikováno v:
Nucleic Acids Research
Bacteriophage λ encodes a DNA recombination system that includes a 5′-3′ exonuclease (λ Exo) and a single strand annealing protein (Redβ). The two proteins form a complex that is thought to mediate loading of Redβ directly onto the single-str
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b505df61636887a5e808551950ae7c2
https://doi.org/10.1093/nar/gky1309
https://doi.org/10.1093/nar/gky1309
Autor:
Felix B. Pedersen, Stan Wang, Marc J. Lajoie, Verena Volf, Xavier Rios, Timothy M. Wannier, Anik Debnath, John Aach, Michael T. Laub, Devon A. Stork, Isaac D. Lutz, Gabriel T. Filsinger, Julie Zhang, Kevin Gozzi, Christopher J. Gregg, Seth L. Shipman, George M. Church, Helene M. Kuchwara
Publikováno v:
Filsinger, G T, Wannier, T M, Pedersen, F B, Lutz, I D, Zhang, J, Stork, D A, Debnath, A, Gozzi, K, Kuchwara, H, Volf, V, Wang, S, Rios, X, Gregg, C J, Lajoie, M J, Shipman, S L, Aach, J, Laub, M T & Church, G M 2021, ' Characterizing the portability of phage-encoded homologous recombination proteins ', Nature Chemical Biology, vol. 17, no. 4, pp. 394-402 . https://doi.org/10.1038/s41589-020-00710-5
Nature chemical biology
Nature chemical biology
Efficient genome editing methods are essential for biotechnology and fundamental research. Homologous recombination (HR) is the most versatile method of genome editing, but techniques that rely on host RecA-mediated pathways are inefficient and labor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ce71405ae8898e68bc616a0a56a0d02
https://portal.findresearcher.sdu.dk/da/publications/a434af18-9d3d-4fe9-be6d-13d7c0ffd667
https://portal.findresearcher.sdu.dk/da/publications/a434af18-9d3d-4fe9-be6d-13d7c0ffd667
Autor:
Jenny M. Tam, George Chao, Adama M. Sesay, Dmitry Ter-Ovanesyan, Raphael Ferreira, Alexander J. Mijalis, Donald E. Ingber, Oliver Dodd, Nina M. Donghia, Erkin Kuru, Lindsey R. Robinson-McCarthy, Joshua Rainbow, Thomas E. Schaus, James J. Collins, Helena de Puig, Robert A. Rasmussen, Gabriel T. Filsinger, Andrew C. Pawlowski, Peng Yin, Timothy M. Wannier, George M. Church, Jeantine E. Lunshof
Publikováno v:
Science
Science, 371(6526), 244-245. AMER ASSOC ADVANCEMENT SCIENCE
Science, 371(6526), 244-245. AMER ASSOC ADVANCEMENT SCIENCE
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5418c1e36845e377eb40bc95fefef1e
Autor:
Ákos Nyerges, Michaela A. Jones, Peter N. Ciaccia, Andrew D. Ellington, Csaba Pál, Kamyab Javanmardi, George M. Church, Farren J. Isaacs, Aditya M. Kunjapur, Gabriel T. Filsinger, Timothy M. Wannier, Max G. Schubert
Publikováno v:
Nat Rev Methods Primers
Nature Reviews Methods Primers
Nature Reviews Methods Primers
Recombination-mediated genetic engineering, also known as recombineering, is the genomic incorporation of homologous single-stranded or double-stranded DNA into bacterial genomes. Recombineering and its derivative methods have radically improved geno
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69844bd7c03c6bd1d7ddb4930e39d3f0
https://doi.org/10.1038/s43586-020-00006-x
https://doi.org/10.1038/s43586-020-00006-x
Autor:
Lindsey Robinson-McCarthy, Alexander J. Mijalis, Gabriel T Filsinger, Helena de Puig, Nina M. Donghia, Thomas E. Schaus, Robert A. Rasmussen, Raphael Ferreira, Jeantine E. Lunshof, George Chao, Dmitry Ter-Ovanesyan, Oliver Dodd, Erkin Kuru, Adama M. Sesay, Joshua Rainbow, Andrew C. Pawlowski, Timothy M. Wannier, Peng Yin, James J. Collins, Donald Ingber, Jenny Tam, George M. Church
The SARS-CoV-2 pandemic has brought about the unprecedented expansion of highly sensitive molecular diagnostics as a primary infection control strategy. At the same time, many laboratories have shifted focus to SARS-CoV-2 research and diagnostic deve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::91e67d0eaf89ca7dd9dcb3f72ee0c6f5
https://doi.org/10.31219/osf.io/9svjq
https://doi.org/10.31219/osf.io/9svjq
Autor:
Stan Wang, Anik Debnath, Marc J. Lajoie, Michael T. Laub, George M. Church, Helene M. Kuchwara, Verena Volf, Gabriel T. Filsinger, Julie Zhang, Kevin Gozzi, Christopher J. Gregg, Timothy M. Wannier, Felix B. Pedersen, John Aach, Devon A. Stork, Seth L. Shipman, Xavier Rios, Isaac D. Lutz
Bacterial genome editing methods are used to engineer strains for biotechnology and fundamental research. Homologous recombination (HR) is the most versatile method of genome editing, but traditional techniques using endogenous RecA-mediated pathways
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::95fd0788133d9c4a0b73a158a203d7c7
Autor:
George M. Church, Csaba Pál, Xavier Rios, Christopher J. Gregg, Marc J. Lajoie, Márton Czikkely, Gabriel T. Filsinger, Helene M. Kuchwara, Timothy M. Wannier, Ákos Nyerges, Nathaniel C. Borders, David Balogh
Publikováno v:
Proc Natl Acad Sci U S A
Exploiting bacteriophage-derived homologous recombination processes has enabled precise, multiplex editing of microbial genomes and the construction of billions of customized genetic variants in a single day. The techniques that enable this, Multiple
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7117f4f7ffa89fe8cd97e18df7a77619
https://doi.org/10.1101/2020.01.14.906594
https://doi.org/10.1101/2020.01.14.906594