Single strand gap repair: The presynaptic phase plays a pivotal role in modulating lesion tolerance pathways.

Autor: Luisa Laureti, Lara Lee, Gaëlle Philippin, Michel Kahi, Vincent Pagès

Publikováno v: PLoS Genetics, Vol 18, Iss 6, p e1010238 (2022)

During replication, the presence of unrepaired lesions results in the formation of single stranded DNA (ssDNA) gaps that need to be repaired to preserve genome integrity and cell survival. All organisms have evolved two major lesion tolerance pathway

Externí odkaz: https://doaj.org/article/52cca27b24524a93897444ac0f6e4178

DNA polymerase IV mediates efficient and quick recovery of replication forks stalled at N2-dG adducts

Autor: Hisaji Maki, Tsutomu Katayama, Mio Ikeda, Gaëlle Philippin, Asako Furukohri, Edward L. Loechler, Masahiro Akiyama, Robert P. P. Fuchs

Publikováno v: Nucleic Acids Research

Escherichia coli DNA polymerase IV (Pol IV, also known as DinB) is a Y-family DNA polymerase capable of catalyzing translesion DNA synthesis (TLS) on certain DNA lesions, and accumulating data suggest that Pol IV may play an important role in copying

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dea72853d0bd70fa61ddee44cb405f62
https://doi.org/10.1093/nar/gku547

Ethylene oxide and propylene oxide derived N7-alkylguanine adducts are bypassed accurately in vivo

Autor: Jean Cadet, Robert P. Fuchs, Gerard Mazón, Gaëlle Philippin, Didier Gasparutto

Publikováno v: DNA Repair
DNA Repair, 2014, 22, pp.133-136. ⟨10.1016/j.dnarep.2014.08.001⟩
DNA Repair, Elsevier, 2014, 22, pp.133-136. ⟨10.1016/j.dnarep.2014.08.001⟩

Adducts formed at the nucleophilic N7 position of guanine are the most abundant lesions produced by alkylating agents such as ethylene oxide (EO) and propylene oxide (PO). In order to investigate the intrinsic mutagenic potential of N 7-alkylguanine

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1da7102247f3da96f1be3afb9e7f4a66
https://cea.hal.science/cea-02074395

Alkyltransferase-like protein (eATL) prevents mismatch repair-mediated toxicity induced by O6-alkylguanine adducts in Escherichia coli

Autor: Mauro Modesti, Robert P. P. Fuchs, Gerard Mazón, Gaëlle Philippin, Jean Cadet, Didier Gasparutto

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, 2010, 107 (42), pp.18050-18055. ⟨10.1073/pnas.1008635107⟩
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2010, 107 (42), pp.18050-18055. ⟨10.1073/pnas.1008635107⟩

O 6 -alkylG adducts are highly mutagenic due to their capacity to efficiently form O 6 -alkylG:T mispairs during replication, thus triggering G→A transitions. Mutagenesis is largely prevented by repair strategies such as reversal by alkyltransferas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d72715b0a054dcf4f4ff2f2a928d357f
https://cea.hal.science/cea-02074463

The alkyltransferase-like ybaZ gene product enhances nucleotide excision repair of O(6)-alkylguanine adducts in E. coli

Autor: Gaëlle Philippin, Jean Cadet, Gerard Mazón, Didier Gasparutto, Robert P. P. Fuchs

Publikováno v: DNA repair. 8(6)

O6-methylguanine adducts are potent pre-mutagenic lesions owing to their high capacity to direct mis-insertion of thymine when bypassed by replicative DNA polymerases. The strong mutagenic potential of these adducts is prevented by alkyltransferases

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fdedc5df37789a30a407488f90081c3
https://pubmed.ncbi.nlm.nih.gov/19269902