Zobrazeno 1 - 10 of 29 pro vyhledávání: '"GOERLICH OTTMAR"'
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Additional file 4 of Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Autor: Mautner, Lena, Christin-Kirsty Baillie, Herold, Heike Marie, Volkwein, Wolfram, Guertler, Patrick, Eberle, Ute, Ackermann, Nikolaus, Sing, Andreas, Pavlovic, Melanie, Goerlich, Ottmar, Busch, Ulrich, Wassill, Lars, Huber, Ingrid, Baiker, Armin
Additional file 4: Figure S3. Specificity of the assay. SARS-CoV Frankfurt 1 RNA and nucleic acid extracts from 20 different samples from respiratory pathogens were tested with RT-LAMP targeting ORF8 (A) and N (B) to determine cross reactivity. For n
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Additional file 1 of Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Autor: Mautner, Lena, Christin-Kirsty Baillie, Herold, Heike Marie, Volkwein, Wolfram, Guertler, Patrick, Eberle, Ute, Ackermann, Nikolaus, Sing, Andreas, Pavlovic, Melanie, Goerlich, Ottmar, Busch, Ulrich, Wassill, Lars, Huber, Ingrid, Baiker, Armin
Additional file 1: Figure S1. Positions of LAMP primers in SARS-CoV-2 genome. LAMP primers of genes ORF8 (A) and N (B) aligned to the SARS-CoV-2 reference sequence. Arrowheads indicate orientation of the primer. Primers F1c and F2 together build the
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::065b3286293595696a2b352cca9d5793
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Additional file 5 of Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Autor: Mautner, Lena, Christin-Kirsty Baillie, Herold, Heike Marie, Volkwein, Wolfram, Guertler, Patrick, Eberle, Ute, Ackermann, Nikolaus, Sing, Andreas, Pavlovic, Melanie, Goerlich, Ottmar, Busch, Ulrich, Wassill, Lars, Huber, Ingrid, Baiker, Armin
Additional file 5: Figure S4. Colorimetric read-out of SARS-CoV-2 RT-LAMP. Positive samples 1–5 and Negative samples 1–5 were pre-heated at 90 °C for 5 min before being directly pipetted into RT-LAMP for SARS-CoV-2 targeting ORF8 and N. As inter
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Additional file 3 of Rapid point-of-care detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Autor: Mautner, Lena, Christin-Kirsty Baillie, Herold, Heike Marie, Volkwein, Wolfram, Guertler, Patrick, Eberle, Ute, Ackermann, Nikolaus, Sing, Andreas, Pavlovic, Melanie, Goerlich, Ottmar, Busch, Ulrich, Wassill, Lars, Huber, Ingrid, Baiker, Armin
Additional file 3: Figure S2. Determination of the sensitivity of the assay. Isolated RNA from SARS-CoV-2 infected Vero cell culture supernatant was quantified using RT-ddPCR and serially diluted to determine the LOD. 1000 copies/µL and 200 copies/
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Verification of analytical methods for GMO testing when implementing interlaboratory validated methods: Version 2
Autor: HOUGS LOTTE, GATTO FRANCESCO, GOERLICH OTTMAR, GROHMANN LUTZ, LIESKE KATHRIN, MAZZARA MARCO, NARENDJA FRANK, OVESNA JAROSLAVA, PAPAZOVA NINA, SCHOLTENS INGRID, ZEL JANA
In the EU, method validation is an essential part of the process that regulates the introduction of new GMOs as food and/or feed into the market. When the inter-laboratory validation study is completed, the method is ready to be implemented in routin
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http://publications.jrc.ec.europa.eu/repository/handle/JRC109940
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