A rapid economical multiplex PCR-RFLP method for molecular detection and genotyping of Giardia duodenalis clinical isolates.

Autor: Ghosal A; Division of Parasitology, ICMR-National Institute of Cholera and Enteric Diseases (ICMR-NICED), Kolkata, India., Sardar SK; Division of Parasitology, ICMR-National Institute of Cholera and Enteric Diseases (ICMR-NICED), Kolkata, India., Haldar T; Division of Parasitology, ICMR-National Institute of Cholera and Enteric Diseases (ICMR-NICED), Kolkata, India., Prasad A; Division of Parasitology, ICMR-National Institute of Cholera and Enteric Diseases (ICMR-NICED), Kolkata, India., Das K; Faculty of Science, Assam Downtown University, Guwahati, Assam 781026, India., Kobayashi S; Department of Parasitology, National Institute of Infectious Diseases (NIID), Tokyo, Japan., Saito-Nakano Y; Department of Parasitology, National Institute of Infectious Diseases (NIID), Tokyo, Japan., Dutta S; Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases (ICMR-NICED), Kolkata, India., Nozaki T; Department of Biomedical Chemistry, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan., Ganguly S; Division of Parasitology, ICMR-National Institute of Cholera and Enteric Diseases (ICMR-NICED), Kolkata, India. Electronic address: sandipanganguly@hotmail.com.

Publikováno v: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2024 Dec; Vol. 110 (4), pp. 116548. Date of Electronic Publication: 2024 Oct 03.

Development of a robust SNP marker set for genotyping diverse gene bank collections of polyploid roses.

Autor: Patzer L; Institute of Plant Genetics, Section Molecular Plant Breeding, Leibniz University Hannover, Hannover, Germany., Thomsen T; Institute of Plant Genetics, Section Molecular Plant Breeding, Leibniz University Hannover, Hannover, Germany., Wamhoff D; Institute of Horticultural Production Systems, Section Woody Plant and Propagation Physiology, Leibniz University Hannover, Hannover, Germany., Schulz DF; Institute of Plant Genetics, Section Molecular Plant Breeding, Leibniz University Hannover, Hannover, Germany.; Current Address: Federal Office of Consumer Protection and Food Safety, Brunswick, Germany., Linde M; Institute of Plant Genetics, Section Molecular Plant Breeding, Leibniz University Hannover, Hannover, Germany., Debener T; Institute of Plant Genetics, Section Molecular Plant Breeding, Leibniz University Hannover, Hannover, Germany. debener@genetik.uni-hannover.de.

Publikováno v: BMC plant biology [BMC Plant Biol] 2024 Nov 14; Vol. 24 (1), pp. 1076. Date of Electronic Publication: 2024 Nov 14.

Genotyping single nucleotide polymorphisms in homologous regions using multiplex kb level amplicon capture sequencing.

Autor: Lu M; College of Biological Science and Medical Engineering, Donghua University, 2999 Renmin north Road, Shanghai, 201620, China., Li J; Department of Emergency, The Second Affiliated Hospital of Air Force Medical University of PLA, Xi'an, Shaanxi, 710038, China., Sun X; College of Biological Science and Medical Engineering, Donghua University, 2999 Renmin north Road, Shanghai, 201620, China., Zhao D; College of Biological Science and Medical Engineering, Donghua University, 2999 Renmin north Road, Shanghai, 201620, China., Zong H; College of Biological Science and Medical Engineering, Donghua University, 2999 Renmin north Road, Shanghai, 201620, China., Tang C; College of Biological Science and Medical Engineering, Donghua University, 2999 Renmin north Road, Shanghai, 201620, China., Li K; College of Biological Science and Medical Engineering, Donghua University, 2999 Renmin north Road, Shanghai, 201620, China., Zhou Y; College of Biological Science and Medical Engineering, Donghua University, 2999 Renmin north Road, Shanghai, 201620, China., Xiao J; College of Biological Science and Medical Engineering, Donghua University, 2999 Renmin north Road, Shanghai, 201620, China. xiaojunhua@dhu.edu.cn.

Publikováno v: Molecular genetics and genomics : MGG [Mol Genet Genomics] 2024 Oct 26; Vol. 299 (1), pp. 99. Date of Electronic Publication: 2024 Oct 26.

The updated mouse universal genotyping array bioinformatic pipeline improves genetic QC in laboratory mice.

Autor: Blanchard MW; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Mutant Mouse Resource and Research Center, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Sigmon JS; Department of Computer Science, University of North Carolina, Chapel Hill, NC 27599, USA.; Mutant Mouse Resource and Research Center, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Brennan J; Mutant Mouse Resource and Research Center, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Ahulamibe C; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Allen ME; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Systems Genetics Core Facility, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Ardery S; Genetics and Molecular Biology Curriculum, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Baric RS; Department of Epidemiology, Gillings School of Public Health, University of North Carolina, Chapel Hill, NC 27599, USA., Bell TA; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Farrington J; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Ciavatta D; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Mutant Mouse Resource and Research Center, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Cruz Cisneros MC; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Genetics and Molecular Biology Curriculum, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Drushal M; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Systems Genetics Core Facility, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Ferris MT; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Systems Genetics Core Facility, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Fry RC; Department of Environmental Sciences and Engineering, Gillings School of Public Health, University of North Carolina, Chapel Hill, NC 27599, USA., Gaines C; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Gu B; Department of Neuroscience, The Ohio State University, Columbus, OH 43210, USA., Heise MT; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Hock P; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Hodges RA; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Systems Genetics Core Facility, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Hulgin M; Systems Genetics Core Facility, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Kafri T; Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27599, USA., Lynch RM; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Magnuson T; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Mutant Mouse Resource and Research Center, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA., Miller DR; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Murphy CEY; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Nguyen DT; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Noll KE; Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC 27599, USA., Proulx MK; Department of Microbiology, UMass Chan Medical School, Worchester, MA 01655, USA., Sassetti CM; Department of Microbiology, UMass Chan Medical School, Worchester, MA 01655, USA., Schoenrock SA; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Shaw GD; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Simon JM; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Smith CM; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA., Styblo M; Department of Nutrition, Gillings School of Public Health, University of North Carolina, Chapel Hill, NC 27599, USA., Tarantino LM; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Woo J; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA., Pardo Manuel de Villena F; Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Mutant Mouse Resource and Research Center, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Systems Genetics Core Facility, Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA.

Publikováno v: G3 (Bethesda, Md.) [G3 (Bethesda)] 2024 Oct 07; Vol. 14 (10).

Optimizing single-tube nested PCR for enhanced genotyping of single cells and in vitro produced bovine embryos.

Autor: Macedo Rafael de Arruda J; Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil., da Silva Mendonça M; Laboratório da Biologia do Reconhecer, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil., Maravilha Braga PV; Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil., Dos Santos Nascimento L; Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil., Burla Dias AJ; Laboratório de Reprodução e Melhoramento Genético Animal, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil., Lopes Rios ÁF; Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil., Magnelli Mangiavacchi P; Laboratório de Reprodução e Melhoramento Genético Animal, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602 Campos dos Goytacazes, RJ, Brazil. Electronic address: paula.magnelli@gmail.com.

Publikováno v: Gene [Gene] 2024 Dec 15; Vol. 929, pp. 148838. Date of Electronic Publication: 2024 Aug 08.