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Publikováno v:
Insect Biochemistry and Molecular Biology. 36:726-734
A partially palindromic 15-nt. sequence upstream from a juvenile hormone-regulated gene (jhp21) was previously identified in the African migratory locust, Locusta migratoria. This sequence was proposed as a juvenile hormone (JH) response element (JHR
Ligand specificity and developmental expression of RXR and ecdysone receptor in the migratory locust
Autor:
G.R. Wyatt, David C. Hayward, Eldon E. Ball, Tarlochan S. Dhadialla, Michael J. Kuiper, Virginia K. Walker, Shutang Zhou
Publikováno v:
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The ecdysone receptor(1), which is a heterodimer of EcR and the retinoic acid receptor (RXR) homolog, Ultraspiracle (USP), has been well studied in the evolutionarily advanced and derived insects, the flies and moths. It is less well characterized in
Autor:
Shutang Zhou, H. Kayser, Jianzhong Zhang, G.R. Wyatt, Yasuo Chinzei, M. Hirai, Virginia K. Walker
Publikováno v:
Molecular and Cellular Endocrinology. 190:177-185
Although juvenile hormone (JH) has essential roles in insect development and reproduction, the molecular mechanisms of gene regulation by JH remain an enigma. In Locusta migratoria, the partially palindromic 15-nt sequence, GAGGTTCGAGA/TCCTT/C, found
Autor:
G.R. Wyatt, Ralph P. Braun
Publikováno v:
Journal of Biological Chemistry. 271:31756-31762
The cDNA for the hexameric hemolymph juvenile hormone-binding protein (JHBP) from the migratory locust has been cloned and sequenced. Antiserum raised against purified JHBP was used to identify clones in an expression library. The 4.3-kilobase JHBP m
Autor:
John B. Ancsin, G.R. Wyatt
Publikováno v:
Insect Biochemistry and Molecular Biology. 26:501-510
Two major hemolymph proteins, belonging to the insect hexameric storage protein family, have been purified from Locusta migratoria . Larval storage protein 1 (LSP1) has M r = 410,000, s = 16.1 S , 10% hexose content and is composed of up to five diff
Publikováno v:
Archives of Insect Biochemistry and Physiology. 32:633-640
When an active JH analog (methoprene or pyriproxyfen) is administered to JH-deprived (precocene-treated) adult female locusts, there is a delay of about 24 h before vitellogenin (Vg), the product of a JH-dependent gene, is found in the hemolymph. If
Autor:
A.V. Glinka, G.R. Wyatt
Publikováno v:
Insect Biochemistry and Molecular Biology. 26:13-18
Transcription of a vitellogenin gene ( VgB ) was measured in nuclei isolated from fat body of adult female locusts, to study its relations to juvenile hormone (JH). During the first ovarian cycle, there was a correlation between the rise and fall in
Publikováno v:
Insect Biochemistry and Molecular Biology. 25:775-781
The suitability of the haemolymph juvenile hormone binding protein (JHBP) of Locusta migratoria for use in a competition assay for juvenile hormone (JH) III has been investigated, and a simple quantitative assay procedure using this protein has been
Publikováno v:
Archives of Insect Biochemistry and Physiology. 28:291-309
Juvenile hormone (JH) binding components from the fat body of the African migratory locust were analyzed in a search for a potential nuclear JH receptor. Biosynthetically prepared 10R[3H]JH III gave a high proportion of specific binding to isolated n