Výsledky vyhledávání - "G.J. Horbach"

Development and validation of a high-content screening in vitro micronucleus assay in CHO-k1 and HepG2 cells

Autor: W.M. Westerink, Tom J.J. Schirris, G.J. Horbach, Willem G.E.J. Schoonen

Publikováno v: Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 724:7-21

In the present study an automated image analysis assisted in vitro micronucleus assay was developed with the rodent cell line CHO-k1 and the human hepatoma cell line HepG2, which are both commonly used in regulatory genotoxicity assays. The HepG2 cel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ed690d83c4431e1c051bd73fbfec87f
https://doi.org/10.1016/j.mrgentox.2011.05.007

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Stable Expression of Human Cytochrome P450 3A4 in Conjunction with Human NADPH-Cytochrome P450 Oxidoreductase in V79 Chinese Hamster Cells

Autor: Wolfgang A. Schmalix, D Lang, G.J. Horbach, R Bocker, P Beaune, C Belloc, Helmut Greim, E.M. de Groene, V Siruguri, A Schneider, B Kleingeist, Johannes Doehmer

Publikováno v: Archives of Biochemistry and Biophysics. 332:295-304

V79 Chinese hamster cells were constructed for stable expression of human cytochrome P450 3A4 with and without coexpression of human NADPH-cytochrome P450 oxidoreductase. Expression of the cDNAs was shown by Northern and Western analyses. Activity wa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::213a131c72bcff79c77ccefb9ca45ef4
https://doi.org/10.1006/abbi.1996.0345

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Transgenic cell lines and changes in gene function and expression: Useful tools in toxicity testing?

Autor: G.J. Horbach, E.M. de Groene

Publikováno v: Toxicology in vitro : an international journal published in association with BIBRA. 9(4)

Molecular biology provides the toxicologist with powerful tools that can be used in the development and use of in vitro techniques in toxicity testing. As an example, a number of cDNAs coding for human cytochrome P-450 enzymes have been cloned, incor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2bb4f8a0fafa32ad0cc3ba9b1b855267
https://pubmed.ncbi.nlm.nih.gov/20650121

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Comparison of three different in vitro mutation assays used for the investigation of cytochrome P450-mediated mutagenicity of nitro-polycyclic aromatic hydrocarbons

Autor: G.J. Horbach, F.M.M. van Och, E.M. de Groene, W.A. Kappers

Publikováno v: Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 2, 466, 143-159

Three different in vitro mutation assays were used to investigate the involvement of cytochrome P450 enzymes in the activation of the nitro- polycyclic aromatic hydrocarbons (nitroPAHs) 1-nitropyrene and 2- nitrofluorene and their reduced metabolites

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f3ab33936154aa59027290310d11411
http://resolver.tudelft.nl/uuid:e8f71e69-615d-46ee-87fc-4acb3f7cb4f6

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Effects of non steroidal anti-inflammatory drugs and sulfonamides on hepatic cytochrome P4502C activity in vitro in goats and cattle

Autor: W.M. Zweers-Zeilmaker, G.J. Horbach, Renger F. Witkamp

Publikováno v: Journal of veterinary pharmacology and therapeutics. 21(2)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0db5e4822815b02675c0f01b7788c36
https://pubmed.ncbi.nlm.nih.gov/9597654

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In vitro complex formation and inhibition of hepatic cytochrome P450 activity by different macrolides and tiamulin in goats and cattle

Autor: G.J. Horbach, W.M. Zweers-Zeilmaker, Renger F. Witkamp, A. S. J. P. A. M. van Miert

Publikováno v: Research in Veterinary Science, 1, 66, 51-55

In humans, clinically relevant drug-drug interactions occur with some macrolide antibiotics via the formation of stable metabolic intermediate (MI) complexes with enzymes of the cytochrome P4503A (CYP3A) subfamily. The formation of such complexes can

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::880f9a54aba39251fe03cd5f920ac561
http://resolver.tudelft.nl/uuid:67317462-f22d-48c7-8fd3-ad9c3fbf0c12

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Differential inhibitory effects of phenytoin, diclofenac, phenylbutazone and a series of sulfonamides on hepatic cytochrome P4502C activity in vitro, and correlation with some molecular descriptors in the dwarf goat (Caprus hircus aegagrus)

Autor: G.J. Horbach, W.M. Zweers-Zeilmaker, Renger F. Witkamp

Publikováno v: Xenobiotica; the fate of foreign compounds in biological systems. 27(8)

1. The aim of the present study was to investigate the potency of various sulfonamides to inhibit tolbutamide hydroxylation (a CYP2C activity) in hepatic microsomal fractions and hepatocytes of the dwarf goat. Also a number of suggested substrates fo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7383b6b8413cd460c269e984b8302fd4
https://pubmed.ncbi.nlm.nih.gov/9293615

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A NIH/3T3 cell line stably expressing human cytochrome P450-3A4 used in combination with a lacZ' shuttle vector to study mutagenicity

Autor: G.J. Horbach, W. Seinen, E.M. de Groene

Publikováno v: European journal of pharmacology. 293(1)

An NIH/3T3 cell line, stably expressing human cytochrome P450-3A4 (CYP3A4) cDNA has been developed. This cell line was used in combination with a shuttle vector, containing the bacterial lacZ' gene as reporter gene, to study mutagenicity. Ethylmethan

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5c623d3e90f678c782569951b1ed1fb
https://pubmed.ncbi.nlm.nih.gov/7545582

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