Zobrazeno 1 - 10
of 70
pro vyhledávání: '"G.A. van Arkel"'
Autor:
C.A.M.J.J. van den Hondel, W. E. Borrias, R.W. van Leen, Adri A. M. Thomas, C.J. Kuhlemeier, A van der Ende, G.A. van Arkel
Publikováno v:
Plasmid, 10(2), 156-163. Academic Press Inc.
We describe the construction of a series of vectors suitable for gene cloning in the cyanobacterium Anacystis nidulans R2. From the indigenous plasmid pUH24, derivatives were constructed with streptomycin as the selective marker; one of these plasmid
Publikováno v:
Nature. 271:417-420
The gene A protein of bacteriophage phiX174 has been used in vitro to convert phiX RFI DNA into the relaxed RFII form by nicking the viral strand. The nucleotide sequence at the 3' end of the nick has been determined as -- T G C T C C C C C A A C T T
Publikováno v:
FEMS Microbiology Letters. 16:7-12
Publikováno v:
Cold Spring Harbor Symposia on Quantitative Biology. 43:331-334
Self-cloning in the cyanobacterium Anacystis nidulans R2: Fate of a cloned gene after reintroduction
Publikováno v:
Plasmid. 14:200-208
Functional analysis of cloned genes often makes use of complementation after introducing these genes into cells of a mutant strain. Problems with this self-cloning step in the cyanobacterium Anacystis nidulans R2 have been encountered, which were mai
Publikováno v:
Molecular and General Genetics MGG. 126:37-51
Pulse-chase and density shift experiments were performed with ϕX174 infected cells. For density shift experiments the cells were first incubated in [14C]thymine containing medium, and after filtration and washing, transferred into [3H]BrdUrd contain
Publikováno v:
Virology, 48(2), 456. Elsevier
The DNA of the 70 S particle of the bacteriophage φX174 has been characterized. By hybridization of RF II DNA of mutants from five different cistrons with DNA from wild-type 70 S particles it is demonstrated that this latter DNA is heterogeneous. Al
Publikováno v:
Virology. 52:408-416
Three independently isolated host range mutants of phage φX174 are localized in gene G. This lends further support to the idea that the gene G product is the main protein involved in the adsorption process. The mapping was performed by hybridizing f
Publikováno v:
Molecular and General Genetics MGG. 105:152-163
Complementation experiments with temperature sensitive (ts) and suppressor sensitive (sus) mutants of bacteriophage ϕX174 unambiguously revealed five cistrons on the basis of a clear bipartition of burst sizes.
Genetic recombination and marker rescue of urea-disrupted bacteriophage T4 in spheroplasts ofE. coli
Publikováno v:
Virology. 13:546-548