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pro vyhledávání: '"G. Van Dessel"'
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Publikováno v:
Biochemical and biophysical research communications
The uptake of FPP and GGPP was studied using primary cultures of chromaffin cells as an experimental model. The translocation of the isoprenyl diphosphates was shown to be both dose- and time-dependent. After incubation and a series of thorough washi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b2a88ec8a760bca3c5dcbcd4c028afe
https://doi.org/10.1006/bbrc.1997.7230
https://doi.org/10.1006/bbrc.1997.7230
Autor:
G, Van Dessel, A, Lagrou
Publikováno v:
Acta biochimica Polonica. 41(3)
A protein catalyzing dolichol transfer between membranes has been purified from bovine liver up to 600-fold by acid precipitation, ammonium sulfate precipitation, ion-exchange chromatography and hydrophobic interaction chromatography. The protein dis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::1c892ef87db215799e07932bc42c184e
https://pubmed.ncbi.nlm.nih.gov/7856402
https://pubmed.ncbi.nlm.nih.gov/7856402
Publikováno v:
Biochimica et biophysica acta
The occurrence of a dolichol transfer factor in LPDS has been demonstrated using three different transfer assays. Applying a three step purification procedure, the transfer factor could be enriched 4000–5000-fold with a recovery of 1–2%. SDS-gel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad6a06906e0e3d6eabf4386590c9b595
https://pubmed.ncbi.nlm.nih.gov/8431493
https://pubmed.ncbi.nlm.nih.gov/8431493
Publikováno v:
Biochimica et biophysica acta
The enzymic conversion of dolichol into dolichoic acid has been studied in bovine thyroid subcellular fractions using [1-3H]dolichol as a substrate. The presence of conversion activity could be demonstrated in both the mitochondrial- and supernatant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c5f1eb5b5dcfe27115832a76c4169a2
https://hdl.handle.net/10067/64620151162165141
https://hdl.handle.net/10067/64620151162165141
Publikováno v:
Biochemistry and cell biology
The kinetics of the oligosaccharide transfer from oligosaccharyl pyrophosphoryldolichol to endogeneous protein acceptors in human fibroblasts were studied. No alterations in the transferase activity and enzyme characteristics could be observed in fib
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81ed3611f83724d5cf77a07856382b50
https://hdl.handle.net/10067/35820151162165141
https://hdl.handle.net/10067/35820151162165141
Publikováno v:
Biochimica et biophysica acta. 1024(1)
Gangliosides (GM1, GT1b, GD3) were incorporated in bovine thyroid plasma membranes using the nonspecific lipid transfer protein from beef liver. The transfer of GT1b or GD3 in the presence of 16 units of transfer protein was twice as high as that of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef5e9013dc52904bcb2653ca3c9f3214
https://pubmed.ncbi.nlm.nih.gov/2337619
https://pubmed.ncbi.nlm.nih.gov/2337619
Publikováno v:
Subcellular Biochemistry ISBN: 9781489916235
Dolichols belong to a family of polymeric lipids with the isoprene unit as the repeating building block. This lipid class represents a relative large group of natural products displaying a wide variety of biological functions (Table I) (Rip et al., 1
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b2e1ff98cc84c7dcf9a4f8b5b5d98fa6
https://doi.org/10.1007/978-1-4899-1621-1_8
https://doi.org/10.1007/978-1-4899-1621-1_8
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 878:338-345
Experimental evidence is presented that a bovine liver pH 5.1 supernatant possesses binding capacity towards dolichol. Optimal binding is found at physiological pH and at 5°C. At higher temperature the binding is drastically reduced. After binding,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5caf7de235f88b772055f155bdc09117
https://doi.org/10.1016/0005-2760(86)90241-9
https://doi.org/10.1016/0005-2760(86)90241-9
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 528:399-408
Four asialo glycolipid fractions have been isolated from bovine thyroid glands. The structures were elucidated by partial hydrolysis, periodate oxidation, permethylation analysis and sequential enzymatic degradation studies. The following structures
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8b0c3f031bd0df65984de5d70e8e863
https://doi.org/10.1016/0005-2760(78)90029-2
https://doi.org/10.1016/0005-2760(78)90029-2