Zobrazeno 1 - 10
of 44
pro vyhledávání: '"G. V. Paddock"'
Publikováno v:
Hepatology (Baltimore, Md.). 21(6)
Studies were performed in Hep3B hepatocytes to better elucidate the mechanisms regulating circulating levels of human group-specific component (Gc). We measured changes in Gc messenger RNA (mRNA) synthesis and levels of secreted protein resulting fro
Publikováno v:
BioTechniques. 15(4)
We have utilized enzyme-linked immunosorbent assay (ELISA) to quantitate PCR-amplified DNA. This method was used to measure mRNA for the vitamin D-binding protein (Gc), beta-actin and the transferrin receptor (TR) gene in the Hep3B cell line. Total R
Autor:
G V, Paddock
Publikováno v:
Methods in enzymology. 217
Autor:
J Abelson, G V Paddock
Publikováno v:
Journal of Biological Chemistry. 250:4207-4219
The nucleotide sequences of species I RNA coded for by bacteriophages T2 and T6 have been analyzed using 32-P-labeled material from T2 and T6-infected cultures of Escherichia coli. The T1 and pancreatic ribonuclease digestion products were partially
Publikováno v:
Journal of Biological Chemistry. 252:3446-3458
Rabbit globin complementary DNA made with RNA-dependent DNA polymerase (reverse transcriptase) was used as a template for in vitro synthesis of 32P-labeled RNA and deoxysubstituted RNA. The sequences of the nucleotides in most of the fragments result
Autor:
J Dorson, Larry McReynolds, Bert W. O'Malley, G V Paddock, John J. Monahan, Robb E. Moses, W Salser, D W Bendure, Savio L. C. Woo
Publikováno v:
Journal of Biological Chemistry. 252:1840-1843
Double-stranded ovalbumin DNA was amplified and purified by the cloning of bacterial transformants. The double-stranded DNA was synthesized from a complete complementary DNA transcript of ovalbumin mRNA using Escherichia coli DNA polymerase I and the
Autor:
Robert J. Linhardt, C. P. Bohl, R. Bender, P. D. Duck, K. Itakura, D. F. Mark, L. S. Lin, S. D. Y. Lu, A. Paau, S. G. Platt, L. Sequeira, G. V. Paddock, A. D. Riggs, H. Rubin, S. M. Weissman, D. Pereira, A. Sood
Publikováno v:
Applied Biochemistry and Biotechnology. 13:75-83
Autor:
L A, McReynolds, J J, Monahan, D W, Bendure, S L, Woo, G V, Paddock, W, Salser, J, Dorson, R E, Moses, B W, O'Malley
Publikováno v:
The Journal of biological chemistry. 252(6)
Double-stranded ovalbumin DNA was amplified and purified by the cloning of bacterial transformants. The double-stranded DNA was synthesized from a complete complementary DNA transcript of ovalbumin mRNA using Escherichia coli DNA polymerase I and the
Publikováno v:
Thymus. 4(6)
Bovine transfer factor (TF)--active in initiating specific responsiveness in human thymus-derived (T) lymphocytes to purified protein derivative from Mycobacterium tuberculosis (PPD) in vitro--was partially purified from the dialyzable portion of med
Autor:
G V, Paddock
Publikováno v:
BioTechniques. 7(8)
In studies of the effects of changes in mRNA structure and sequence on the initiation of protein synthesis, we used a generally applicable approach to transcribe reconstructed genes for duck alpha A globin by the bacteriophage SP6 RNA polymerase prom