Zobrazeno 1 - 6
of 6
pro vyhledávání: '"G. P. Malenko"'
Publikováno v:
Reproduction, Fertility and Development. 31:142
A prospective method of vitrification in triacetate cellulose hollow fibres (HF) introduced by Matsunari et al. (2012 J. Reprod. Dev. 58, 599-608) allowed significant simplification and standardization of vitrification/warming procedures and was succ
Publikováno v:
Biology Bulletin. 37:446-452
The dynamics of in vitro development of reconstructed bovine embryos activated at various time after electrofusion was studied. The in vitro mature oocytes without zona pellucida enucleated using the blind method were taken as cytoplasts. Fetal fibro
Improving the stages of bovine cloning technology with the use of oocytes without the zona pellucida
Autor:
T. A. Antipova, M. V. Pinyugina, M. I. Prokof’ev, O. I. Stepanov, A. V. Komissarov, G. P. Malenko
Publikováno v:
Russian Agricultural Sciences. 34:340-342
Bovine embryos are reconstructed with the use of fetal fibroblasts as donors of nuclei and enucleated oocytes without the zona pellucida as recipients. The stages of electrofusion and in vitro culturing of embryos without the zone pellucida up to the
Publikováno v:
Izvestiia Akademii nauk. Seriia biologicheskaia. (5)
The dynamics of in vitro development of reconstructed embryos of cattle after various electrofusion times was studied. The in vitro mature oocytes without zona pellucida enucleated using the touch method were taken as cytoplasts. Fetal fibroblasts we
Publikováno v:
Izvestiia Akademii nauk. Seriia biologicheskaia. (3)
Cloned bovine embryos were produced at the blastocyst stage. Prior to enucleation, oocytes were freed from the zona pellucida. Fibroblasts isolated from the bovine fetus were used as nuclear donors. Pairs of fetal fibroblasts and enucleated oocytes (
Autor:
M. I. Prokofiev, O. I. Stepanov, M. V. Pinyugina, A. V. Komissarow, G. P. Malenko, T. A. Antipova
Publikováno v:
Reproduction, Fertility and Development. 19:156
In animal cloning, recipient oocytes are commonly enucleated in metaphase II (MII). By removal of the first polar body (PB1) and 20–30% of the adjacent cytoplasm, without chromatin staining (blind enucleation), only 60% of bovine oocytes can be enu