Zobrazeno 1 - 10
of 20
pro vyhledávání: '"G. P. Kishchenko"'
Autor:
N.N. Karpishev, Petrenko Va, G. P. Kishchenko, Akimenko Za, Lev S. Sandakhchiev, Eroshkin Am, A.A. Ilyichev, V.I. Ofitzerov, Minenkova Oo, Tat'kov Si
Publikováno v:
FEBS Letters. 301:322-324
Foreign DNA fragments were inserted into filamentous phage gene VIII to create hybrid B-proteins with foreign sequences in the amino terminus. The hybrid proteins are incorporated into the virions which retain viability and infectivity. Virions with
Autor:
S I, Tat'kov, O Iu, Smirnova, N O, Tsivkovskaia, G P, Kishchenko, A A, Il'ichev, V A, Petrenko, L S, Sandakhchiev
Publikováno v:
Doklady Akademii nauk. 346(3)
Autor:
O O, Minenkova, A A, Il'ichev, G P, Kishchenko, T N, Il'icheva, Iu L, Khripin, S F, Oreshkova, V A, Petrenko
Publikováno v:
Molekuliarnaia biologiia. 27(3)
Earlier we developed an expression vector on the basis of bacteriophage M13 allowing the exposure of short peptides on the virion surface. It was used to obtain a recombinant phage carrying the antigenic determinant of HIVI gag protein p17. This phag
Publikováno v:
Molekuliarnaia biologiia. 25(6)
We have studied the virion structure of M13 strains (M13B1, M13BOM1, M13BOM2, M13BOL1) with chimeric variants of B-protein. Data concerning the spatial structure of chimeric B-protein molecules and their interaction with intraphage DNA were obtained.
[A method of equalizing the level of DNA sequences of varying amount in a heterogeneous DNA mixture]
Publikováno v:
Molekuliarnaia biologiia. 25(5)
A method for the equalization of double-stranded DNA concentrations in the mixture which may be used for equalizing double-stranded cDNA concentrations involves thermal denaturation of the double-stranded DNA mixture followed by reassociation. The in
Autor:
I A, Davidovich, G P, Kishchenko
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (6)
The survival curves for bacteriophage lambda and vaccinia virus were shown theoretically and in experiments to have a plateau at prolonged inactivation by UV-irradiation or 8-methoxypsoralen. The level of the plateau is dependent of the accuracy of t
Autor:
G P, Kishchenko
Publikováno v:
Biofizika. 35(5)
The following model of aging is proposed: 1) defective proteins with anomalous primary structures are synthesized sometimes; 2) these defective proteins are precipitated in cells and intercellular spaces; 3) the precipitated proteins block them up un
Autor:
G P, Kishchenko
Publikováno v:
Biofizika. 35(4)
Effect of the length of DNA internucleosome regions (L) on writhing number (Wr) was analysed. It has been shown that Wr(L) is a periodic function. In any period Wr decreases, if L increases. The process of chromatin activation is proposed. The value
Publikováno v:
Laboratornoe delo. (11)
The following procedure is proposed: fixation for 12 h in low ionic strength solution (4 percent formaldehyde in 50 mM sodium phosphate buffer), drying of the suspension drop on the slide, gallocyanin staining. All red cells were contrast-stained wit
Autor:
A. D. Gruzdev, G. P. Kishchenko
Publikováno v:
Biophysics of Structure and Mechanism. 4:97-110
The molecules of the fluorescent dye acridine orange (AO) bind to DNA in such a way that the absorption and emission dipoles lie on a plane perpendicular to the DNA axis. For this reason, definite fluorescence polarization should correspond to each m