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Publikováno v:
FEMS Microbiology Reviews. 24:429-448
Flow cytometry has become a valuable tool in aquatic and environmental microbiology that combines direct and rapid assays to determine numbers, cell size distribution and additional biochemical and physiological characteristics of individual cells, r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f605f60d818528256fd5b1f63d0ae79
https://doi.org/10.1111/j.1574-6976.2000.tb00549.x
https://doi.org/10.1111/j.1574-6976.2000.tb00549.x
Publikováno v:
Journal of Microbiological Methods. 31:113-125
This report describes the use of the fluorescent probe, 5(6)-carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), to determine pHi in growing cells of the spoilage yeast Saccharomyces cerevisiae. The technique is based on the pH-dependent intra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::80c93f0e7a26d9e233c5e641b66f8493
https://doi.org/10.1016/s0167-7012(97)00095-x
https://doi.org/10.1016/s0167-7012(97)00095-x
Autor:
G. Nebe-von Caron, R. A. Badley
Publikováno v:
Journal of Microscopy. 179:55-66
SUMMARY In microbiology cells are called viable when they can be shown to proliferate. Due to long lag phases and the cells' sensitivity to the conditions around them, this definition presents problems when investigating stressed or injured cells. Co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6c6d554be45d5516e27788d5e8acc6ce
https://doi.org/10.1111/j.1365-2818.1995.tb03612.x
https://doi.org/10.1111/j.1365-2818.1995.tb03612.x
Publikováno v:
Journal of microbiological methods. 66(1)
Listeria monocytogenes has previously been shown to adapt to a wide variety of environmental niches, principally those associated with low pH, and this compromises its control in food environments. An understanding of the mechanism(s) by which L. mon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86cde1936db2545d2dbde7192685e58d
https://pubmed.ncbi.nlm.nih.gov/16316702
https://pubmed.ncbi.nlm.nih.gov/16316702
Autor:
C J, Hewitt, G, Nebe-Von-Caron
Publikováno v:
Cytometry. 44(3)
When using traditional microbiological techniques to monitor cell proliferation and viability, stressed, sublethally injured, or otherwise "viable but nonculturable" cells often go undetected. Because of this, such cells often are not considered by m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e3c0654e3b87a1b056d509cee60d158f
https://pubmed.ncbi.nlm.nih.gov/11429768
https://pubmed.ncbi.nlm.nih.gov/11429768
Publikováno v:
International journal of food microbiology. 60(2-3)
Previously, Stephens et al. [J. Appl. Microbiol. 83 (1997) 445-455] developed a sensitive technique for studying the resuscitation of low levels of stressed Salmonella. Using this technique the influence on recovery performance of the peptone compone
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e06ab14f750e47d55a4571f51bb631e
https://pubmed.ncbi.nlm.nih.gov/11016616
https://pubmed.ncbi.nlm.nih.gov/11016616
Publikováno v:
Biotechnology and bioengineering. 70(4)
Multiparameter flow cytometric techniques developed in our laboratories have been used for the "at-line" study of fed-batch bacterial fermentations. These fermentations were done at two scales, production (20 m(3)) and bench (5 x 10(-3) m(3)). In add
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6b99a2b7a8f907809142e6da72015edc
https://pubmed.ncbi.nlm.nih.gov/11005920
https://pubmed.ncbi.nlm.nih.gov/11005920
Publikováno v:
Journal of applied microbiology. 84(6)
Rapid bacterial detection and viability measurements have been greatly enhanced by recent advances in the use of fluorescent stains in cytometry. It has previously been shown that four physiological states can be distinguished: reproductively viable,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2e7703f0f08367323848818f7eb9356
https://pubmed.ncbi.nlm.nih.gov/9717283
https://pubmed.ncbi.nlm.nih.gov/9717283
Publikováno v:
Letters in applied microbiology. 25(4)
Flow cytometry in combination with fluorescent molecular markers 5- (and 6-) carboxyfluorescein succinimidylester (CFSE) and propidium iodide (PI) have been applied to determine lag times, numbers of cell divisions and injury after mild heat (50 degr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e3de7c85e43f161ca39a1f90db2b318
https://pubmed.ncbi.nlm.nih.gov/9351280
https://pubmed.ncbi.nlm.nih.gov/9351280