Zobrazeno 1 - 10
of 35
pro vyhledávání: '"G. Mauras"'
Publikováno v:
Parasitology Research. 76:563-565
Two methods were compared for the isolation of the Toxoplasma gondii pellicle: centrifugation using a discontinuous sucrose gradient and affinity purification by microspheres activated with protein A. Electron microscopy showed that these two means o
Publikováno v:
Parasitology. 114
The protozoan parasite Toxoplasma gondii possesses a triple surface membrane called the pellicle. This is made of an outer plasmalemma and an inner membrane complex lying under the plasmalemma. Using a high salt glycerol treatment followed by sonicat
Publikováno v:
Bulletin de l'Association des anatomistes. 78(240)
Toxoplasma gondii membrane complex was fragmented by sonication and separated on a sucrose continuous gradient. Ultrastructural analysis of the 1,08 g/cm3 density fraction (that which corresponded with a maximal detection by the monoclonal antibody p
Publikováno v:
Annales de parasitologie humaine et comparee. 57(1)
We have demonstrated resistant forms of Trichomonas vaginalis studied their morphology and induced transformation into vegetative forms. This work has led to an hypothesis concerning the parasite cycle which may throw light on the problems of transmi
Publikováno v:
Biomedicine / [publiee pour l'A.A.I.C.I.G.]. 27(1)
Publikováno v:
Annales d'immunologie. (2)
The authors describe a method for isolation of Toxoplasma gondii antigenic membrane components. Technical work is described into four points: 1) specific labelling of membrane proteins was realised by DD125ISA; 2) methacrylate microspheres were bound
Publikováno v:
Biomedicinepharmacotherapy = Biomedecinepharmacotherapie. 40(3)
Tachyzoites of Toxoplasma gondii have been radioiodinated under various conditions with or without lactoperoxidase, with glucose oxidase being used to generate hydrogen peroxide. Erythrocytes were iodinated simultaneously as a control. In our conditi
Publikováno v:
Biomedicine / [publiee pour l'A.A.I.C.I.G.]. 25(6)
2640 sera were titered simultaneously with IHA and IFAT. The results obtained show a good correlation between the two technics used. The IHA test seems particularly useful in IgM detection and, according to the mode of operation, is able to detect lo
Publikováno v:
Biomedicine / [publiee pour l'A.A.I.C.I.G.]. 25(5)
Three different sorts of antigen are prepared ultrasonically: a soluble endogenous antigen, an insoluble particular antigen and a total mixed antigen. Formolinized red blood cells are sensibilized with glutaraldehyde. The authors study the sensitivit
Publikováno v:
Biomedicinepharmacotherapy = Biomedecinepharmacotherapie. 36(2)
Purification procedures of Toxoplasma gondii suspensions are described. These methods were designed for the elimination of leucocytes or TG 180 cells. We have been using either gradient centrifugation in Ficoll or gel filtration. In the first case, o