Zobrazeno 1 - 10
of 33
pro vyhledávání: '"G. M. Gaucher"'
Autor:
M. J. Rollins, G. M. Gaucher
Publikováno v:
Applied Microbiology and Biotechnology. 41:447-455
In this study the addition of ammonium ions (5-30 mM) to Penicillium urticae shake-flask cultures before, during and after the onset of polyketide biosynthesis was examined in a time-dependent manner for its repressive effect on metabolites and a mar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ac2ea5b6abce62add1c74c3d810fd4f
https://doi.org/10.1007/bf00939034
https://doi.org/10.1007/bf00939034
Autor:
M. J. Rollins, G. M. Gaucher
Publikováno v:
Applied Microbiology and Biotechnology. 41:447-455
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::77f6d7b39e2e4dfe426ffd7bd715bb18
https://doi.org/10.1007/bf00212256
https://doi.org/10.1007/bf00212256
Publikováno v:
Canadian journal of microbiology. 37(1)
A 7.7-kilobase (kb) Penicillium urticae genomic DNA fragment containing the 3′ terminus of the 6-methylsalicylic acid polyketide synthetase gene was cloned using a 41-mer mixed oligodeoxynucleotide probe which was based on a cyanogen bromide cleava
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f265147ff0cf5faf5c4c040c258a01b
https://pubmed.ncbi.nlm.nih.gov/2021899
https://pubmed.ncbi.nlm.nih.gov/2021899
Publikováno v:
Biotechnology Letters. 5:785-790
Production of the mycotoxin, patulin, by immobilized cells ofPenicillium urticae was chosen as a model system to study the formation of secondary metabolites on a long term basis. Spores of the fungus were immobilized in carrageenan beads and allowed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::722f514c3d313af81b00d5a514c185f2
https://doi.org/10.1007/bf01386648
https://doi.org/10.1007/bf01386648
Autor:
G. M. Gaucher, Y. M. Deo
Publikováno v:
Biotechnology Letters. 5:125-130
Conidia of Penicillium urticae, immobilized in κ-carrageenan beads, were germinated in situ to form a patulin producing cell mass by incubating these beads in a growth supporting medium. When these in situ grown immobilized cells were repeatedly tra
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8f4f5567c8806bbb3772d0f99f6ef6d9
https://doi.org/10.1007/bf00132171
https://doi.org/10.1007/bf00132171
Publikováno v:
Canadian Journal of Microbiology. 32:259-267
The effect of trace metal nutrition on the functioning of the patulin biosynthetic pathway in submerged cultures of Penicillium urticae (NRRL 2159A) was examined by both chromatographic and enzymological means. Comprehensive metal ion analysis showed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fde2525b4f1b5eec3d3f1812e245d5f
https://doi.org/10.1139/m86-051
https://doi.org/10.1139/m86-051
Publikováno v:
Biotechnology Letters. 5:573-578
Relatively high levels of nutrients and low levels of waste products were maintained in cultures of hybridoma cells by dialysis against growth medium supplemented with fetal calf serum (FCS). This resulted in a greater than ten fold increase in cell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::882bb32c6c05e3a22a84f83765c956af
https://doi.org/10.1007/bf00130835
https://doi.org/10.1007/bf00130835
Publikováno v:
Canadian Journal of Microbiology. 32:167-175
m-Hydroxybenzylalcohol dehydrogenase (EC 1.1.1.97), a secondary metabolism associated protein from stationary phase cultures of Penicillium urticae, was stabilized in crude extracts prior to purification. Stabilization studies resulted in the formula
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00c9bd526297fb9fc5c53ea6987d7904
https://doi.org/10.1139/m86-033
https://doi.org/10.1139/m86-033
Publikováno v:
Canadian Journal of Biochemistry. 53:298-307
The inactivation of highly purified papain (2 × 10−5 M in distilled water) by hydrogen peroxide, generated by the γ-irradiation of water, was examined. The kinetics of activity loss at 23 °C was second order (k = 3.7 × 103 M−1 min−1) for pa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c3d15836449ff8bc73a102be983ad79
https://doi.org/10.1139/o75-042
https://doi.org/10.1139/o75-042
Autor:
Kenneth J. Stevenson, G M Gaucher
Publikováno v:
Biochemical Journal. 151:527-542
The specificity of thermomycolase toward glucagon and the oxidized A and B chains of insulin was investigated. Extensive digestion of glucagon occurred when conducted at pH 7.0 and 45 degrees C for 40 min, whereas hydrolysis of only three peptide bon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::beb83d34a947ae9ed507b8579284bdb4
https://doi.org/10.1042/bj1510527
https://doi.org/10.1042/bj1510527