Zobrazeno 1 - 10
of 183
pro vyhledávání: '"G. M. Clore"'
Publikováno v:
Journal of biomolecular NMR. 66(2)
We present a simple, convenient and robust protocol for expressing perdeuterated proteins in E coli BL21(DE3) cells in shaker flasks that reduces D2O usage ten-fold and d7-glucose usage by 30%. Using a modified M9 medium and optimized growth conditio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f62ee42efcee6a5da6f794c0cd1a4f7
https://pubmed.ncbi.nlm.nih.gov/27709314
https://pubmed.ncbi.nlm.nih.gov/27709314
Autor:
Yu Wai Chen, G M Clore
Publikováno v:
Acta Crystallographica Section D Biological Crystallography. 56:1535-1540
Molecular replacement using search models derived from nuclear magnetic resonance (NMR) spectroscopy has often proved problematic. It has been known for some time that the overall differences in atomic positions (r.m.s.d.) between the crystalline and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a7bf0dfec259885f28e4302eb9af9c8
https://doi.org/10.1107/s0907444900012002
https://doi.org/10.1107/s0907444900012002
Publikováno v:
Nature Structural Biology. 6:868-875
In the Gag-Pol polyprotein of HIV-1, the 99-amino acid protease is flanked at its N-terminus by a transframe region (TFR) composed of the transframe octapeptide (TFP) and 48 amino acids of the p6pol, separated by a protease cleavage site. The intact
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::95f8f1a75892dc18787439b105a70778
https://doi.org/10.1038/12327
https://doi.org/10.1038/12327
Publikováno v:
Nature Structural Biology. 6:166-173
The solution structure of the first protein-protein complex of the bacterial phosphoenolpyruvate: sugar phosphotransferase system between the N-terminal domain of enzyme I (EIN) and the histidine-containing phosphocarrier protein HPr has been determi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::05a060dadb173989ff35780037a8f25e
https://doi.org/10.1038/5854
https://doi.org/10.1038/5854
Publikováno v:
Journal of Molecular Biology. 277:605-620
The solution structure of a complex between the DNA binding domain of a fungal GATA factor and a 13 base-pair oligonucleotide containing its physiologically relevant CGATAG target sequence has been determined by multidimensional nuclear magnetic reso
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::115819c4d8c69a6b60da1a504dfa4ede
https://doi.org/10.1006/jmbi.1998.1625
https://doi.org/10.1006/jmbi.1998.1625
Autor:
Herbert N. Arst, Mats Wikström, Angela M. Gronenborn, G M Clore, S Schumacher, Mary R. Starich
Publikováno v:
Journal of Molecular Biology. 277:621-634
The seemingly innocuous leucine-to-valine mutation at position 22 of the AREA DNA binding domain results in dramatic changes in the in vivo expression profile of genes controlled by this GATA transcription factor. This is associated with a preference
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cd04b1ae4e66099030bcb25cf6688ad2
https://doi.org/10.1006/jmbi.1997.1626
https://doi.org/10.1006/jmbi.1997.1626
Publikováno v:
Protein Science. 7:789-793
The phosphorylated form of the N-terminal domain of enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system of Escherichia coli has been investigated by one-bond and long-range 1H-15N correlation spectroscopy. The active site His 189 is p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::479712bdb2016bbad1b57a3afcb58396
https://doi.org/10.1002/pro.5560070329
https://doi.org/10.1002/pro.5560070329
Publikováno v:
Journal of Biomolecular NMR. 11:97-102
A cost-effective protocol for uniform 15N and/or 13C isotope labeling of bacterially expressed proteins is presented. Unlike most standard protocols, cells are initially grown in a medium containing nutrients at natural abundance and isotopically lab
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a2a962b70cf20276bc0aada478b166bb
https://doi.org/10.1023/a:1008222131470
https://doi.org/10.1023/a:1008222131470
Publikováno v:
Nature Structural Biology. 4:567-577
The solution structure of the N-terminal zinc binding domain (residues 1-55; IN1-55) of HIV-1 integrase has been solved by NMR spectroscopy. IN1-55 is dimeric, and each monomer comprises four helices with the zinc tetrahedrally coordinated to His 12,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11547f8442142452e5cc39808aef3772
https://doi.org/10.1038/nsb0797-567
https://doi.org/10.1038/nsb0797-567
Publikováno v:
Trends in Biochemical Sciences. 21:302-308
Mammalian male sex determination is controlled by a complex hierarchy of gene regulatory proteins and hormones, which promote male gonadal development and regression of the female primordia. At the core of this pathway lies the SRY protein, the maste
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0e0e2fcca442656a930ab29010d1abca
https://doi.org/10.1016/0968-0004(96)10032-3
https://doi.org/10.1016/0968-0004(96)10032-3