Use of the GRP1 PH domain as a tool to measure the relative levels of PtdIns(3,4,5)P3 through a protein-lipid overlay approach

Autor: Hervé Guillou, Charlotte Lécureuil, Karen E. Anderson, Sabine Suire, G. John Ferguson, Chris D. Ellson, Alexander Gray, Nullin Divecha, Phillip T. Hawkins, Len R. Stephens

Publikováno v: Journal of Lipid Research, Vol 48, Iss 3, Pp 726-732 (2007)

We describe a novel approach to the relative quantification of phosphatidylinositol (3,4,5)-trisphosphate [PtdIns(3,4,5)P3] and its application to measure, in neutrophils, the activation of phosphoinositide 3-kinase (PI3K). This protein-lipid overlay

Externí odkaz: https://doaj.org/article/2b2c2d886e3941b18e8415ca8495f34b

Proteomic analysis in primary T cells reveals IL-7 alters T cell receptor thresholding via CYTIP/cytohesin/LFA-1 localisation and activation

Autor: Rayner M. L. Queiroz, Siân C. Piper, Johanna S. Rees, Sam Strickson, Emmanuel Briend, Choon Pei Low, G. John Ferguson, Kathryn S. Lilley, Antony P. Jackson, Donna K. Finch

The ability of the cellular immune system to discriminate self from foreign antigens depends on the appropriate calibration of the T cell receptor (TCR) signalling threshold. The lymphocyte homeostatic cytokine interleukin 7 (IL-7) is known to affect

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01bd18a14737c71474063278e134f316
https://www.repository.cam.ac.uk/handle/1810/332866

SCFAs induce mouse neutrophil chemotaxis through the GPR43 receptor.

Autor: Marco A R Vinolo, G John Ferguson, Suhasini Kulkarni, George Damoulakis, Karen Anderson, Mohammad Bohlooly-Y, Len Stephens, Phillip T Hawkins, Rui Curi

Publikováno v: PLoS ONE, Vol 6, Iss 6, p e21205 (2011)

Short chain fatty acids (SCFAs) have recently attracted attention as potential mediators of the effects of gut microbiota on intestinal inflammation. Some of these effects have been suggested to occur through the direct actions of SCFAs on the GPR43

Externí odkaz: https://doaj.org/article/b81bd6ce141845e09edfc122af8ac149

Localizing the lipid products of PI3Kγ in neutrophils

Autor: Arnaud Deladeriere, Hervé Guillou, Sabine Suire, Oliver Rausch, G. John Ferguson, Simon Andrews, Simon Walker, Tamara Chessa, Till Bretschneider, Cheng-Jin Du, Laura J. Norton, Anne Segonds-Pichon, Len R. Stephens, Takehiko Sasaki, Peter Finan, Yvonne E. Lindsay, Phillip T. Hawkins, John M. Lucocq

Publikováno v: Advances in Biological Regulation
Advances in Biological Regulation, 2016, 60, pp.36-45. ⟨10.1016/j.jbior.2015.10.005⟩
Advances in Biological Regulation (60), 36-45. (2016)

Class I phosphoinositide 3-kinases (PI3Ks) are important regulators of neutrophil migration in response to a range of chemoattractants. Their primary lipid products PtdIns(3,4,5)P3 and PtdIns(3,4)P2 preferentially accumulate near to the leading edge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81ebb156550e35ea5bae9fafd142bb50

IL-18 associated with lung lymphoid aggregates drives IFNγ production in severe COPD

Autor: Katherine Stephenson, Michiko Mori, Sanjay Sethi, Christine K. Ward, Jonas S. Erjefält, Natasha A. Karp, Gautam Damera, Donna K. Finch, Emmanuel Briend, G. John Ferguson, Matthew A. Sleeman

Publikováno v: Respiratory Research

Background Increased interferon gamma (IFNγ) release occurs in Chronic Obstructive Pulmonary Disease (COPD) lungs. IFNγ supports optimal viral clearance, but if dysregulated could increase lung tissue destruction. Methods The present study investig

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::699e845eb61d284adf3a9d0154da472a
https://doi.org/10.1186/s12931-017-0641-7