Zobrazeno 1 - 10
of 32
pro vyhledávání: '"G. Idini"'
Publikováno v:
FEMS Microbiology Letters. 202:45-50
Mycoplasma agalactiae, the causative agent of contagious agalactia in small ruminants, produces a protein, named P80, that is detectable in all wild-type isolates examined to date and that appears expressed during the early phase of infection. We des
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c90381271b3a8e5333f0ec99bf553e3
https://doi.org/10.1111/j.1574-6968.2001.tb10778.x
https://doi.org/10.1111/j.1574-6968.2001.tb10778.x
Autor:
Sebastiana Tola, Stefano Rocca, Guido Leori, G. Idini, Angela Maria Rocchigiani, Daniela Manunta
Publikováno v:
Veterinary Microbiology. 80:121-130
We have constructed a physical map of the Mycoplasma agalactiae strain PG2 chromosome analyzing it by pulsed field gel electrophoresis in a contour-clamped homogeneous electric-field system. We mapped 33 cleavage sites generated with SmaI, XhoI, SalI
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa7e2bd0c74e72f3cd6f7dc1522b8ac9
https://doi.org/10.1016/s0378-1135(00)00389-8
https://doi.org/10.1016/s0378-1135(00)00389-8
Autor:
M. Cocco, Angela Maria Rocchigiani, Guido Leori, P. P. Angioi, G. Idini, Stefano Rocca, Sebastiana Tola, Daniela Manunta
Publikováno v:
Journal of Veterinary Medicine Series B. 46:199-206
Mycoplasma agalactiae and Mycoplasma bovis are closely related species in phylogenetic terms. Pulsed Field Gel Electrophoresis (PFGE) of SmaI, EclXI, Bsi WI, MluI, BssHII, SalI, XhoI, NruI and ApaI digested DNAs were used to analyse and to compare re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6db267053daf460cf85071229a9bdf2c
https://doi.org/10.1046/j.1439-0450.1999.00226.x
https://doi.org/10.1046/j.1439-0450.1999.00226.x
Autor:
Guido Leori, A. Angioi, G. Idini, Sebastiana Tola, Angela Maria Rocchigiani, Daniela Manunta, Grazia Galleri
Publikováno v:
Veterinary Microbiology. 51:77-84
A polymerase chain reaction (PCR)-based test was developed for the detection of Mycoplasma agalactiae in sheep milk samples. Two oligonucleotide primers were designed to amplify a 375 bp fragment of M. agalactiae chromosomal DNA. Amplified products w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8de1b818935392f5d4e9dda87ba486aa
https://doi.org/10.1016/0378-1135(96)00023-5
https://doi.org/10.1016/0378-1135(96)00023-5
Publikováno v:
Journal of Phytopathology. 132:265-272
An indirect Dot-ELISA was compared with DAS-ELISA for detecting Artichoke Latent Virus (ALV) both in purified preparations and in crude sap from “Spinoso sardo” artichoke leaves. Antigen diluted samples (1 μl) were first spotted on nitrocellulos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ba29ab17d16f4bc4bd08bf468960d654
https://doi.org/10.1111/j.1439-0434.1991.tb00121.x
https://doi.org/10.1111/j.1439-0434.1991.tb00121.x
Autor:
Carla Longheu, Toniangelo Onni, G. Idini, Marisa Fusco, Sebastiana Tola, Elisabetta Marras, Lucia Corona
Publikováno v:
Clinical and vaccine immunology : CVI. 14(4)
We developed a new recombinant enzyme-linked immunosorbent assay (rELISA) for serodiagnosis of contagious agalactia (CA), a disease caused by Mycoplasma agalactiae in sheep and goats. The assay is based on two M. agalactiae surface proteins, namely,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4028fa2bc6afa0762266c6463c6e0556
https://pubmed.ncbi.nlm.nih.gov/17287317
https://pubmed.ncbi.nlm.nih.gov/17287317
Publikováno v:
Molecular and cellular probes. 19(3)
The membrane-protein 81 gene (mb-mp81) of Mycoplasma bovis was cloned, sequenced and compared to membrane-protein 81 gene (ma-mp81) of Mycoplasma agalactiae. After alignment of both sequences, specific primers pairs were designed from variable or unc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::623e04130f1b643775eadaf8c32f0855
https://pubmed.ncbi.nlm.nih.gov/15797822
https://pubmed.ncbi.nlm.nih.gov/15797822
Autor:
Guido Leori, Sebastiana Tola, Angela Maria Rocchigiani, G. Idini, Daniela Manunta, P. P. Angioi, Stefano Rocca
Publikováno v:
Vaccine. 17(22)
Five sets of vaccines were prepared using Mycoplasma agalactiae washed cultures inactivated with phenol (1), formalin (2), heat-treatment (3), sodium hypochlorite (4) and saponin (5). All sets, except for saponin-vaccine, were adjuvated with aluminiu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c02fcb4276669dd5887b1757a39fd0f
https://pubmed.ncbi.nlm.nih.gov/10438045
https://pubmed.ncbi.nlm.nih.gov/10438045
Autor:
Franco Turrini, Antonio Angioi, Guido Leori, Daniela Manunta, Angela Maria Rocchigiani, G. Idini, Sebastiana Tola, Monica Cocco
Publikováno v:
FEMS microbiology letters. 154(2)
We have analyzed antigenic variation of seven M. agalactiae wild strains using different sera from naturally infected sheep. Only 30 day sera recognized all surface proteins and inhibited the growth of mycoplasmas. Furthermore, we have observed that
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bbf3dc4bb4ac849030b9cfd36077dd4f
https://pubmed.ncbi.nlm.nih.gov/9311134
https://pubmed.ncbi.nlm.nih.gov/9311134
Autor:
Grazia Galleri, Angela Maria Rocchigiani, G. Idini, Antonio Angioi, Daniela Manunta, Sebastiana Tola, Guido Leori
Publikováno v:
Veterinary microbiology. 54(1)
We developed a simple and rapid method for DNA extraction from sheep milk to use for polymerase chain reaction (PCR) diagnosis of Mycoplasma agalactiae. We tested 357 samples from 21 newly infected flocks (group 1) and 87 samples from 8 flocks infect
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3104339370b73061ee712e0cad7f00fc
https://pubmed.ncbi.nlm.nih.gov/9050167
https://pubmed.ncbi.nlm.nih.gov/9050167