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Modified monoclonal antibody production kinetics kappa/gamma mRNA levels, and metabolic activities in a murine hybridoma selected by continuous Culture

Autor: H. Keller, E. Couve, M. Leno, L. Cabanié, O.-W. Merten, G. E. Palfi, D. Moeurs

Publikováno v: Biotechnology and Bioengineering. 44:753-764

During long-term continuous culture of the hybridoma cell line 11317, a better-producing subclone (I1317-SF11), giving improved productivity, has been selected. The comparison of the original cell line (I1317-DC) with this subclone revealed that alth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::771242d4cf6a174450cd7f36a56a0679
https://doi.org/10.1002/bit.260440612

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Modified monoclonal antibody production kinetics kappa/gamma mRNA levels, and metabolic activities in a murine hybridoma selected by continuous Culture

Autor: O W, Merten, D, Moeurs, H, Keller, M, Leno, G E, Palfi, L, Cabanié, E, Couvé

Publikováno v: Biotechnology and bioengineering. 44(6)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::36b78cd7e8fa300dd46c5bc7706c2f80
https://pubmed.ncbi.nlm.nih.gov/18618836

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A new method for the encapsulation of mammalian cells

Autor: O W, Merten, H, Dautzenberg, G E, Palfi

Publikováno v: Cytotechnology. 7(2)

A new encapsulation method was developed for the cultivation of mammalian cells. The capsules were produced using a solution of sodium cellulose sulphate (CS)(1.5%) and poly-dimethyl-diallyl-ammonium chloride (PDMDAAC). When CS droplets fell into the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ab3afbab3d1499facc170f0eda838d80
https://pubmed.ncbi.nlm.nih.gov/1367907

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On-line determination of biochemical/physiological parameters in the fermentation of animal cells in a continuous or discontinuous mode

Autor: O W, Merten, G E, Palfi, J, Steiner

Publikováno v: Advances in biotechnological processes. 6

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f537a8c0c25fdb8f0459858d8bfb8af7
https://pubmed.ncbi.nlm.nih.gov/3535830

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Invasive infrared sensor for the determination of the cell number in a continuous fermentation of hybridomas

Autor: O W, Merten, G E, Palfi, J, Stäheli, J, Steiner

Publikováno v: Developments in biological standardization. 66

A new and simple device for the on-line determination of the total cell count in fermentation vessels is presented. It is based on fibre optic techniques, produces a constant infrared signal and determines the cell count via the scattered light. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2ea5357a792b49cca04b0e9331f58276
https://pubmed.ncbi.nlm.nih.gov/3582764

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