Zobrazeno 1 - 10
of 377
pro vyhledávání: '"G. Deluca"'
Publikováno v:
STAR Protocols, Vol 3, Iss 4, Pp 101915- (2022)
Summary: There are challenges to using commercially available antibodies generated in animals, including concerns with reproducibility, high costs, and ethical issues. Here, we present a protocol for generating and purifying recombinant antibodies fr
Externí odkaz:
https://doaj.org/article/57792dc217874db382413c74c76753ca
Autor:
Keith F DeLuca, Jeanne E Mick, Amy H Ide, Wanessa C Lima, Lori Sherman, Kristin L Schaller, Steven M Anderson, Ning Zhao, Timothy J Stasevich, Dileep Varma, Jakob Nilsson, Jennifer G DeLuca
Publikováno v:
eLife, Vol 10 (2021)
Antibodies are indispensable tools used for a large number of applications in both foundational and translational bioscience research; however, there are drawbacks to using traditional antibodies generated in animals. These include a lack of standard
Externí odkaz:
https://doaj.org/article/702303f606c44e00b209f26179885455
Autor:
Robert T. Wimbish, Jennifer G. DeLuca
Publikováno v:
Frontiers in Cell and Developmental Biology, Vol 8 (2020)
Successful mitotic cell division is critically dependent on the formation of correct attachments between chromosomes and spindle microtubules. Microtubule attachments are mediated by kinetochores, which are large proteinaceous structures assembled on
Externí odkaz:
https://doaj.org/article/354f7c974a8d4f3380a94cbfd25646f6
Publikováno v:
Biology Open, Vol 8, Iss 6 (2019)
The spindle assembly checkpoint (SAC) prevents erroneous chromosome segregation by delaying mitotic progression when chromosomes are incorrectly attached to the mitotic spindle. This delay is mediated by mitotic checkpoint complexes (MCCs), which ass
Externí odkaz:
https://doaj.org/article/15a7b33c6c2a4beba094c9d82c8d7803
Publikováno v:
Molecular Biology of the Cell.
During mitosis, kinetochore-microtubule attachments are monitored by a molecular surveillance system known as the spindle assembly checkpoint. The prevailing model posits that dynein evicts checkpoint proteins (e.g., Mad1, Mad2) from stably attached
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::35f2e11620d975b14679c484e516a3c5
https://doi.org/10.1091/mbc.e23-04-0130
https://doi.org/10.1091/mbc.e23-04-0130
Akademický článek
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Akademický článek
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Autor:
Jun Zhu, Patrick Paddison, Raymund L. Yong, Jennifer G. DeLuca, Chad M. Toledo, Jacob A. Herman, Huaien Wang, Margaret Pain, Eunjee Lee
Supplementary Data includes Supplementary Materials and Methods (cell culture protocols, methods for predicting BUB1BR/S status and extracting molecular profiles of GBM tumor cells, genome-wide shRNA screens, GBM cohort data, drug sensitivity measure
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43c492e3a90395c3a9a10ed422c061af
https://doi.org/10.1158/0008-5472.22415405.v1
https://doi.org/10.1158/0008-5472.22415405.v1
Autor:
Jun Zhu, Patrick Paddison, Raymund L. Yong, Jennifer G. DeLuca, Chad M. Toledo, Jacob A. Herman, Huaien Wang, Margaret Pain, Eunjee Lee
Glioblastoma multiforme (GBM) remains a mainly incurable disease in desperate need of more effective treatments. In this study, we develop evidence that the mitotic spindle checkpoint molecule BUB1B may offer a predictive marker for aggressiveness an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::57d608196ef47372d22a5bd11c999c6e
https://doi.org/10.1158/0008-5472.c.6509276.v1
https://doi.org/10.1158/0008-5472.c.6509276.v1
Autor:
Jacob A. Herman, Romario R. Romain, Pia Hoellerbauer, Hazheen K. Shirnekhi, David C. King, Keith F. DeLuca, Erin Osborne Nishimura, Patrick J. Paddison, Jennifer G. DeLuca
Publikováno v:
Proceedings of the National Academy of Sciences. 119
Aneuploidy, the incorrect number of whole chromosomes, is a common feature of tumors that contributes to their initiation and evolution. Preventing aneuploidy requires properly functioning kinetochores, which are large protein complexes assembled on
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9976595bc94cf2157e3e8588e62cf097
https://doi.org/10.1073/pnas.2208255119
https://doi.org/10.1073/pnas.2208255119