Zobrazeno 1 - 10
of 41
pro vyhledávání: '"G. Brett Robb"'
Autor:
Kristin A. Fluke, Ryan T. Fuchs, Yueh-Lin Tsai, Victoria Talbott, Liam Elkins, Hallie P. Febvre, Nan Dai, Eric J. Wolf, Brett W. Burkhart, Jackson Schiltz, G. Brett Robb, Ivan R. Corrêa, Thomas J. Santangelo
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-20 (2024)
Abstract RNAs are often modified to invoke new activities. While many modifications are limited in frequency, restricted to non-coding RNAs, or present only in select organisms, 5-methylcytidine (m5C) is abundant across diverse RNAs and fitness-relev
Externí odkaz:
https://doaj.org/article/af268ebdcd824d6989e97d95df96dc37
Autor:
Ryan T. Fuchs, Jennifer L. Curcuru, Megumu Mabuchi, Audrey Noireterre, Peter R. Weigele, Zhiyi Sun, G. Brett Robb
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-16 (2022)
Two distinct Cas12a orthologs (Cme and Yme) from metagenomic sources are characterized. The enzymes are found to be thermotolerant and are compared to three mesophilic Cas12a enzymes (Lba, Fno and Asp).
Externí odkaz:
https://doaj.org/article/2f051ab7df9a4bb69aca89664afee82f
Autor:
Giedrius Gasiunas, Joshua K. Young, Tautvydas Karvelis, Darius Kazlauskas, Tomas Urbaitis, Monika Jasnauskaite, Mantvyda M. Grusyte, Sushmitha Paulraj, Po-Hao Wang, Zhenglin Hou, Shane K. Dooley, Mark Cigan, Clara Alarcon, N. Doane Chilcoat, Greta Bigelyte, Jennifer L. Curcuru, Megumu Mabuchi, Zhiyi Sun, Ryan T. Fuchs, Ezra Schildkraut, Peter R. Weigele, William E. Jack, G. Brett Robb, Česlovas Venclovas, Virginijus Siksnys
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available to
Externí odkaz:
https://doaj.org/article/eff8b92a7b8248269794dd870fd5600c
Small RNA Expression Profiling by High-Throughput Sequencing: Implications of Enzymatic Manipulation
Publikováno v:
Journal of Nucleic Acids, Vol 2012 (2012)
Eukaryotic regulatory small RNAs (sRNAs) play significant roles in many fundamental cellular processes. As such, they have emerged as useful biomarkers for diseases and cell differentiation states. sRNA-based biomarkers outperform traditional messeng
Externí odkaz:
https://doaj.org/article/abf94485bc81469381a4004008572d80
Autor:
Kathleen A. Christie, Jimmy A. Guo, Rachel A. Silverstein, Roman M. Doll, Megumu Mabuchi, Hannah E. Stutzman, Jiecong Lin, Linyuan Ma, Russell T. Walton, Luca Pinello, G. Brett Robb, Benjamin P. Kleinstiver
Publikováno v:
Nature Biotechnology. 41:409-416
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d76210e861acf8181c3af196e0bc046a
https://doi.org/10.1038/s41587-022-01492-y
https://doi.org/10.1038/s41587-022-01492-y
Autor:
S. Hong Chan, Joseph M. Whipple, Nan Dai, Theresa M. Kelley, Kathryn Withers, George Tzertzinis, Ivan R. Corrêa, G. Brett Robb
Publikováno v:
RNA. 28:1144-1155
Advances in mRNA synthesis and lipid nanoparticles technologies have helped make mRNA therapeutics and vaccines a reality. The 5’ cap structure is a crucial modification required to functionalize synthetic mRNA for efficient protein translation in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ed0a9974423db24ac6db5fef1e94c07
https://doi.org/10.1261/rna.079173.122
https://doi.org/10.1261/rna.079173.122
Autor:
Tomas Urbaitis, Giedrius Gasiunas, Joshua K Young, Zhenglin Hou, Sushmitha Paulraj, Egle Godliauskaite, Mantvyda M Juskeviciene, Migle Stitilyte, Monika Jasnauskaite, Megumu Mabuchi, G Brett Robb, Virginijus Siksnys
Publikováno v:
EMBO reports, Hoboken : Wiley, 2022, vol. 23, iss. 12, art. no. e55481, p. [1-15]
Most CRISPR-type V nucleases are stimulated to cleave double-stranded (ds) DNA targets by a T-rich PAM, which restricts their targeting range. Here, we identify and characterize a new family of type V RNA-guided nuclease, Cas12l, that exclusively rec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ad27a91f59b7039c4602433f8e2cbba
https://pubmed.ncbi.nlm.nih.gov/36268581
https://pubmed.ncbi.nlm.nih.gov/36268581
Akademický článek
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Autor:
Justin Albers, Megumu Mabuchi, Ryan T. Fuchs, Prarthana Mohanraju, Richard van Kranenburg, Jennifer L. Curcuru, Ioannis Mougiakos, G. Brett Robb, John van der Oost
Publikováno v:
CRISPR Journal, 4(1), 82-91
CRISPR J
CRISPR Journal 4 (2021) 1
CRISPR J
CRISPR Journal 4 (2021) 1
The ability of CRISPR-Cas12a nucleases to function reliably in a wide range of species has been key to their rapid adoption as genome engineering tools. However, so far, Cas12a nucleases have been limited for use in organisms with growth temperatures
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef649b486c311acffb3f3104cae4722f
https://doi.org/10.1089/crispr.2020.0086
https://doi.org/10.1089/crispr.2020.0086
Autor:
Kathleen A. Christie, Jimmy A. Guo, Rachel A. Silverstein, Roman M. Doll, Megumu Mabuchi, Hannah E. Stutzman, Linyuan Ma, G. Brett Robb, Benjamin P. Kleinstiver
While restriction enzymes (REs) remain the gold-standard for manipulating DNA in vitro, they have notable drawbacks including a dependence on short binding motifs that constrain their ability to cleave DNA substrates. Here we overcome limitations of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::90883b1070d6818cc6bf7499320c9c40
https://doi.org/10.1101/2022.01.11.474553
https://doi.org/10.1101/2022.01.11.474553
