Zobrazeno 1 - 10
of 17
pro vyhledávání: '"G Yu, Lomakina"'
Autor:
G. Yu. Lomakina, N. N. Ugarova
Publikováno v:
Moscow University Chemistry Bulletin. 77:42-47
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::32db9615d7a039f7b25e5565626e4cec
https://doi.org/10.3103/s0027131422010059
https://doi.org/10.3103/s0027131422010059
Publikováno v:
Moscow University Chemistry Bulletin. 75:374-381
The kinetics of the hydrolysis of ATP by apyrase A from the Solanum tuberosum potato is studied at a pH of 6.5 and 25°C by the bioluminescent method. KM = 33 μM and V = 0.37 μM/s are determined for ATP. It is shown that the hydrolysis of ATP proce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::866f377f32c7e650978ab0c985a92c6c
https://doi.org/10.3103/s0027131420060139
https://doi.org/10.3103/s0027131420060139
Publikováno v:
Moscow University Chemistry Bulletin. 75:186-194
Digitonin (monodesmoid saponin) forms complexes with cholesterol of cell membranes, causing the disintegration of the membrane structure, the formation of pores, and the release of intracellular components in the reaction medium. We use HEK293 cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::41982330075d8d12ea82c7002064a391
https://doi.org/10.3103/s0027131420030086
https://doi.org/10.3103/s0027131420030086
Autor:
N.N. Ugarova, G. Yu. Lomakina
Publikováno v:
Moscow University Chemistry Bulletin. 75:15-21
The distinctive features of firefly luciferase bioluminescence are complex changes in the shape of the spectra and λmax of bioluminescence with varying pH, temperature, and enzyme structure. An analysis of the published data and the authors’ own r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::085a80e2adf7b1fdb3b07160557b610e
https://doi.org/10.3103/s0027131420010113
https://doi.org/10.3103/s0027131420010113
Publikováno v:
Moscow University Chemistry Bulletin. 74:191-197
The Bacille Calmette–Guerine (BCG) vaccine, which is based on a live strain of Mycobacterium bovis BCG is widely used for the immunoprophylaxis of tuberculosis. One of the BCG vaccine’s key parameters is its сell viability (specific activity: th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::222f35d67d856a81991accb463ec7397
https://doi.org/10.3103/s0027131419040084
https://doi.org/10.3103/s0027131419040084
Autor:
G. Yu. Lomakina, N. G. Balabushevich, Olga Pobeguts, T. V. Vakhrusheva, M A Moshkovskaya, E. V. Mikhalchik, Yu. P. Baikova, P L Shcherbakov, E A Sholina, S. A. Gusev
Publikováno v:
Bulletin of Experimental Biology and Medicine. 165:235-238
Cells of E. coli isolates from the gut of healthy volunteers (N=5) and patients with Crohn's disease (N=5) and laboratory E. coli strain DH5α bound mucin in vitro in similar amounts ranging from 0.02 to 0.12 mg/mg of bacterial dry weight. Binding wa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2067c144c8174ed7950a727db435995d
https://doi.org/10.1007/s10517-018-4137-3
https://doi.org/10.1007/s10517-018-4137-3
Autor:
G. Yu. Lomakina, S. S. Aleksandrova, O. V. Podobed, D. V. Grishin, N. S. Melik-Nubarov, I. D. Grozdova, O. Yu. Abakumova, M. V. Pokrovskaya, Nikolay N. Sokolov, V. S. Pokrovski
Publikováno v:
Applied Biochemistry and Microbiology. 53:165-172
N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from Erwinia carotovora. A method of L-asparaginase modification by the obtained activat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4d8924a54c80efd41db628e81f9a5c42
https://doi.org/10.1134/s0003683817020119
https://doi.org/10.1134/s0003683817020119
Autor:
Konstantin M. Boyko, S. A. Yakimov, Eugene G. Maksimov, Lada E. Petrovskaya, Mikhail P. Kirpichnikov, Dmitry A. Dolgikh, Vladimir Popov, M.V. Kryukova, E. A. Kryukova, G. Yu. Lomakina
Publikováno v:
Biomolecules
Volume 9
Issue 12
Volume 9
Issue 12
PMGL3 is a cold-adapted esterase which was recently isolated from the permafrost metagenomic library. It exhibits maximum activity at 30 °
C and low stability at elevated temperatures (40 °
C and higher). Sequence alignment has reveal
C and low stability at elevated temperatures (40 °
C and higher). Sequence alignment has reveal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::839ad05cba5f5f9f2011837d038dfbc9
https://pubmed.ncbi.nlm.nih.gov/31888238
https://pubmed.ncbi.nlm.nih.gov/31888238
Autor:
N S, Melik-Nubarov, I D, Grozdova, G Yu, Lomakina, M V, Pokrovskaya, V S, Pokrovski, S S, Aleksandrova, O Yu, Abakumova, O V, Podobed, D V, Grishin, N N, Sokolov
Publikováno v:
Prikladnaia biokhimiia i mikrobiologiia. 53(2)
N-hydroxysuccinimide ester of monomethoxy polyethylene glycol hemisuccinate was synthesized. It acylated amino groups in a molecule of recombinant L-asparaginase from Erwinia carotovora. A method of L-asparaginase modification by the obtained activat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::443b609dfcfdad27ee24fb572d2aee01
https://pubmed.ncbi.nlm.nih.gov/29508977
https://pubmed.ncbi.nlm.nih.gov/29508977
Publikováno v:
Moscow University Chemistry Bulletin. 69:49-55
A method has been developed for synthesizing stable and highly active conjugates of firefly luciferase with antibodies using the heterobifunctional coupling agent N-succinimidyl 3-(2-pyridyldithio) propionate linking NH2 groups of antibodies with fre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b197454350a0aec0509494995e27c023
https://doi.org/10.3103/s0027131414020047
https://doi.org/10.3103/s0027131414020047