Zobrazeno 1 - 10
of 11
pro vyhledávání: '"G I, Lepesheva"'
Publikováno v:
Biochemistry. Biokhimiia. 66(5)
Cytochrome P450-dependent monooxygenases, in spite of their wide distribution, can be simply divided into a few groups differing in the location of the electron transfer chain and their composition. The two main groups of cytochrome P450-dependent mo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::1e57a264e3d894704f05fa750f69cc16
https://pubmed.ncbi.nlm.nih.gov/11405894
https://pubmed.ncbi.nlm.nih.gov/11405894
Publikováno v:
Biochemistry. Biokhimiia. 65(12)
Our previous chemical modification and cross-linking studies identified some positively charged amino acid residues of cytochrome P450scc that may be important for its interaction with adrenodoxin and for its functional activity. The present study wa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::fa86962b92e6e48782bc8b47b502a8b3
https://pubmed.ncbi.nlm.nih.gov/11173513
https://pubmed.ncbi.nlm.nih.gov/11173513
Publikováno v:
Biochemistry. Biokhimiia. 64(9)
The conditions for heterologous expression of recombinant bovine adrenodoxin in E. coli have been optimized, thus reaching expression levels up to 12-14 micromoles per liter of culture medium. A highly efficient method for purification of this recomb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::00365c8883788cac3a85f9e15185255b
https://pubmed.ncbi.nlm.nih.gov/10521726
https://pubmed.ncbi.nlm.nih.gov/10521726
Autor:
G I, Lepesheva, S A, Usanov
Publikováno v:
Biochemistry. Biokhimiia. 63(2)
Optimization of the conditions for heterologous expression of recombinant cytochrome P450scc in E. coli provided an expression level of about 420 nmoles of cytochrome P450scc per liter of bacterial culture. A new procedure for purification of recombi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a9de2575b06c8213ea60e64bced7c718
https://pubmed.ncbi.nlm.nih.gov/9526119
https://pubmed.ncbi.nlm.nih.gov/9526119
Autor:
G I, Lepesheva, S A, Usanov
Publikováno v:
Biochemistry. Biokhimiia. 62(6)
Studies have been done to assess the effect of selective chemical modification of Lys338 on the functional activity and conformational mobility of cytochrome P450scc. It is found that fluorescently labelled cytochrome P450scc retains cholesterol side
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3de1793d349a74403e17b683463dae51
https://pubmed.ncbi.nlm.nih.gov/9324423
https://pubmed.ncbi.nlm.nih.gov/9324423
Autor:
G I, Lepesheva, S A, Usanov
Publikováno v:
Biokhimiia (Moscow, Russia). 61(8)
Clinical modification of cytochrome P450scc with fluoresceine isothiocyanate (FITC) results in selective incorporation of the fluorescent label into Lys338. According to affinity chromatography on immobilized adrenodoxin, fluorescently labeled cytoch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::bc35307e8bd5280622aa6ffa535ec690
https://pubmed.ncbi.nlm.nih.gov/8962914
https://pubmed.ncbi.nlm.nih.gov/8962914
Publikováno v:
Biokhimiia (Moscow, Russia). 59(7)
The possibilities of direct antigen detection in unlabelled systems based on immunoglobulin G Langmuir-Blodgett films as a sensitive surface have been studied. It was shown that an increase in the monolayer number in an immunoglobulin G Langmuir-Blod
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::bc8888c8f3fc860569bdbe14e0f4c235
https://pubmed.ncbi.nlm.nih.gov/7948419
https://pubmed.ncbi.nlm.nih.gov/7948419
Publikováno v:
Biokhimiia (Moscow, Russia). 58(6)
Modification of rabbit IgG by coproporphyrin I activated by Woodward's reagent K as well as antigen-binding and fluorescent properties of coproporphyrin-IgG have been studied. It was shown that coproporphyrin I modified IgG retains its capacity to bi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::dd2c9fd8260cf62040418b1922e306f7
https://pubmed.ncbi.nlm.nih.gov/8364116
https://pubmed.ncbi.nlm.nih.gov/8364116
Autor:
G I, Lepesheva, S P, Martsev
Publikováno v:
Biokhimiia (Moscow, Russia). 57(7)
Since immunoglobulins are used in a vast variety of immunoassays, the problem of obtaining antibodies with enhanced antigen-binding activity is of great importance. In order to discriminate between putative approaches to activating antibody modificat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8d52a803bcca9808089ac301961b853a
https://pubmed.ncbi.nlm.nih.gov/1391214
https://pubmed.ncbi.nlm.nih.gov/1391214
Autor:
G I, Lepesheva, S P, Martsev
Publikováno v:
Zhurnal mikrobiologii, epidemiologii i immunobiologii. (10)
The possibility of improving analytical parameters of the immunometric assay with the use of biotinylated antibodies and biotin-streptavidin complexes in comparison with the commonly known approach of direct antibody modification with 125I has been s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::04953f8a01b61f5b6d462172fe8d43e8
https://pubmed.ncbi.nlm.nih.gov/2075757
https://pubmed.ncbi.nlm.nih.gov/2075757