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From genes to proteins: high-throughput expression and purification of the human proteome

Autor: J S, Albala, K, Franke, I R, McConnell, K L, Pak, P A, Folta, B, Rubinfeld, A H, Davies, G G, Lennon, R, Clark

Publikováno v: Journal of cellular biochemistry. 80(2)

The development of high-throughput methods for gene discovery has paved the way for the design of new strategies for genome-scale protein analysis. Lawrence Livermore National Laboratory and Onyx Pharmaceuticals, Inc., have produced an automatable sy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a198e16a697072abf7b951325309e0be
https://pubmed.ncbi.nlm.nih.gov/11074586

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Identification of seven new human MHC class I region genes around the HLA-F locus

Autor: W, Fan, W, Cai, S, Parimoo, D C, Schwarz, G G, Lennon, S M, Weissman

Publikováno v: Immunogenetics. 44(2)

Using cDNA hybridization selection techniques, we identified seven new genes in a 280 kilobase YAC covering the HLA-F locus. The new genes were mapped back to the YAC by a combination of optical restriction mapping and pulse field gel electrophoresis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c090952a33cbe27d153fa54f5505be3
https://pubmed.ncbi.nlm.nih.gov/8662070

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Sequence and expression of a candidate for the human Secretor blood group alpha(1,2)fucosyltransferase gene (FUT2). Homozygosity for an enzyme-inactivating nonsense mutation commonly correlates with the non-secretor phenotype

Autor: R J, Kelly, S, Rouquier, D, Giorgi, G G, Lennon, J B, Lowe

Publikováno v: The Journal of biological chemistry. 270(9)

Synthesis of soluble A, B, H, and Lewis b blood group antigens in humans is determined by the Secretor (Se) (FUT2) blood group locus. Genetic, biochemical, and molecular analyses indicate that this locus corresponds to an alpha(1,2)fucosyltransferase

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d0a59b23e47e521652f626d0124fd157
https://pubmed.ncbi.nlm.nih.gov/7876235

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Molecular cloning of a human genomic region containing the H blood group alpha(1,2)fucosyltransferase gene and two H locus-related DNA restriction fragments. Isolation of a candidate for the human Secretor blood group locus

Autor: S, Rouquier, J B, Lowe, R J, Kelly, A L, Fertitta, G G, Lennon, D, Giorgi

Publikováno v: The Journal of biological chemistry. 270(9)

We have used the human H blood group alpha(1,2)fucosyltransferase (FUT1) cDNA to screen chromosome 19 cosmid libraries in a search for the human Secretor (Se) blood group gene (FUT2). One cosmid has been isolated that contains two distinct segments t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::775f3f6f2a13734ddda8b3113fa876e6
https://pubmed.ncbi.nlm.nih.gov/7876234

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Use of high coverage reference libraries of Drosophila melanogaster for relational data analysis. A step towards mapping and sequencing of the genome

Autor: J D, Hoheisel, G G, Lennon, G, Zehetner, H, Lehrach

Publikováno v: Journal of molecular biology. 220(4)

Three differently made, primary Drosophila cosmid libraries of 16-fold genome coverage have been generated. Also, a jumping library has been created by a new method that takes advantage of methylation differences between genomic DNA and vector. Third

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::45ce49a6b8bd5f77155f27b73322a6b2
https://pubmed.ncbi.nlm.nih.gov/1908905

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Easy removal of oil from microtiter dishes following PCR

Autor: G G, Lennon, R, Drmanac, H, Lehrach

Publikováno v: BioTechniques. 11(2)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::82638ba823af988c9e4fe20de95cf6e1
https://pubmed.ncbi.nlm.nih.gov/1931015

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The temporal order of appearance of transcripts from unrearranged and rearranged Ig genes in murine fetal liver

Autor: G G, Lennon, R P, Perry

Publikováno v: Journal of immunology (Baltimore, Md. : 1950). 144(5)

The developmental time course of RNA transcribed from unrearranged (germ-line) and rearranged Ig genes in murine fetal liver was determined by a quantitative Northern blot analysis. Sterile Cmu transcripts and germ-line VH transcripts are detectable

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::91ebc61a03b80cec20466971a53d4b68
https://pubmed.ncbi.nlm.nih.gov/2106560

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