Zobrazeno 1 - 9
of 9
pro vyhledávání: '"G B Collier"'
Autor:
J. A. MacLeod, Dan Wang, W. C. Dicke, Jared J. Drader, A. C. Nemeth, S. Manalili Wheeler, James C. Hannis, G. B. Collier
Publikováno v:
Lab on a Chip. 16:2513-2520
Point of care molecular diagnostics benefits from a portable battery-operated device capable of performing a fast turnaround using reliable inexpensive cartridges. We describe a prototype device for performing a molecular diagnostics test for clinica
Autor:
G P Livi, M Angelichio, K R Gibson, J F Connaughton, G B Collier, P J Fleming, P G Vanek, W D Kaloss
Publikováno v:
Journal of Biological Chemistry. 268:9490-9495
To characterize the mechanism of membrane attachment of dopamine beta-hydroxylase, an expression system producing the processed form of this enzyme has been developed. We have replaced the endogenous signal peptide of bovine dopamine beta-hydroxylase
Autor:
L S, Bazar, G B, Collier, P G, Vanek, B A, Siles, Y W, Kow, P W, Doetsch, R P, Cunningham, J G, Chirikjian
Publikováno v:
Electrophoresis. 20(6)
We introduce a novel experimental strategy for DNA mutation detection named the Mismatch Identification DNA Analysis System (MIDAS) [1, 2], which has an associated isothermal probe amplification step to increase target DNA detection sensitivity to at
Publikováno v:
Journal of capillary electrophoresis. 5(1-2)
The detection of base pair mismatches in limiting amounts of DNA is important in the early diagnosis of cancer and other genetic diseases. The specific type and exact location of a bp mismatch in certain genes can yield information on the likelihood
Autor:
B A, Siles, G B, Collier
Publikováno v:
Journal of capillary electrophoresis. 3(6)
A low-viscosity, polysaccharide matrix (TreviSol-CE [TS-CE], Trevigen, Inc., Gaithersburg, MD, U.S.A.) has been characterized experimentally in capillary electrophoresis for the separation of DNA fragments. The mass fraction (%) of the matrix in buff
Publikováno v:
Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society. 6(1)
TreviGel-500, a new polysaccharide matrix containing AgaCryl, commercially available as a powder for slab gel electrophoresis, is now being applied to the separation of DNA fragments in capillary electrophoresis. The capillary mode allows the use of
Publikováno v:
BioTechniques. 18(4)
In this paper we outline a simplified protocol for the electrophoretic mobility shift assay utilizing TreviGel 500, a nontoxic alternative to polyacrylamide. The TreviGel 500 matrix combines the strength and resolution of polyacrylamide with the simp
Publikováno v:
Gene. 148(1)
To study the effects of in vivo DNA methylation, we have developed an inducible system to control the intracellular concentration of S-adenosyl-L-methionine (AdoMet). The product of the bacteriophage T3 AdoMet hydrolase-encoding gene (amh), which deg
Autor:
K R, Gibson, P G, Vanek, W D, Kaloss, G B, Collier, J F, Connaughton, M, Angelichio, G P, Livi, P J, Fleming
Publikováno v:
The Journal of biological chemistry. 268(13)
To characterize the mechanism of membrane attachment of dopamine beta-hydroxylase, an expression system producing the processed form of this enzyme has been developed. We have replaced the endogenous signal peptide of bovine dopamine beta-hydroxylase