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Isolation of monocytes from whole blood by density gradient centrifugation and counter-current elutriation followed by cryopreservation: six years' experience

Autor: A B Westvik, Per Kr Lund, Reidun Øvstebø, G B Joø, Peter Kierulf

Publikováno v: Scandinavian Journal of Clinical and Laboratory Investigation. 60:357-366

Monocyte purification by means of counter-current elutriation and subsequent cryopreservation for future use was initiated in 1986 and has been established as a routine since 1993.To sum up and evaluate our method for the isolation and preservation o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f722b41cc78b20de58b1afe61f2b9fe
https://doi.org/10.1080/003655100750019260

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Net inflammatory capacity of human septic shock plasma evaluated by a monocyte-based target cell assay: identification of interleukin-10 as a major functional deactivator of human monocytes

Autor: P. Kierulf, Petter Brandtzaeg, A B Westvik, R. Ovstebo, G B Joø, L. T. N. Osnes

Publikováno v: Journal of Experimental Medicine. 184:51-60

We have developed a functional assay to study the inflammatory capacity of plasma collected from patients with severe gram-negative septic shock. In this assay, elutriation-purified, cryo-preserved human monocytes from one healthy donor are combined

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::9b4ef1b2ac88895ef1ec5875199a5d38
https://doi.org/10.1084/jem.184.1.51

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Lipopolysaccharide activation of human monocytes mediated by CD14, results in a coordinated synthesis of tissue factor, TNF-α and IL-6

Autor: C. Okkenhaug, Peter Kierulf, Åse-Brit Westvik, Reidun Øvstebø, G-B. Joø, L. T. N. Osnes

Publikováno v: Journal of Endotoxin Research. 2:27-35

We have investigated the role of CD14 in lipopolysaccharide (LPS) stimulation of monocytes to tissue factor (TF) synthesis, tumor necrosis factor (TNF-α) antigen and interleukin-6 (IL-6) antigen release. Elutriation-purified monocytes or peripheral

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ef3b7dedabc01610954cf22f90c7198c
https://doi.org/10.1177/096805199500200104

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Neisseria meningitidis lipopolysaccharides in human pathology

Autor: P, Brandtzaeg, A, Bjerre, R, Øvstebø, B, Brusletto, G B, Joø, P, Kierulf

Publikováno v: Journal of endotoxin research. 7(6)

Neisseria meningitidis causes meningitis, fulminant septicemia or mild meningococcemia attacking mainly children and young adults. Lipopolysaccharides (LPS) consist of a symmetrical hexa-acyl lipid A and a short oligosaccharide chain and are classifi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3afe2a5f27ff23841f7c64ee17d93a87
https://pubmed.ncbi.nlm.nih.gov/11753210

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Aspirin potentiates LPS-induced fibrin formation (FPA) and TNF-alpha-synthesis in whole blood

Autor: L T, Osnes, K B, Haug, G B, Joø, A B, Westvik, R, Ovstebø, P, Kierulf

Publikováno v: Thrombosis and haemostasis. 83(6)

The effect of aspirin on LPS-incubation of whole blood was investigated. Aspirin induced a concentration dependent increase (2.5-5-fold at 5 mM aspirin) in LPS-induced appearance of TNF-alpha and fibrinopeptide A (FPA) in plasma, despite the concomit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::8755ed2b5c947ebe6ee01ffdadffc250
https://pubmed.ncbi.nlm.nih.gov/10896240

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Acetylsalicylic acid and sodium salicylate inhibit LPS-induced NF-kappa B/c-Rel nuclear translocation, and synthesis of tissue factor (TF) and tumor necrosis factor alfa (TNF-alpha) in human monocytes

Autor: L T, Osnes, K B, Foss, G B, Joø, C, Okkenhaug, A B, Westvik, R, Ovstebø, P, Kierulf

Publikováno v: Thrombosis and haemostasis. 76(6)

We have investigated the effects of acetylsalicylic acid and sodium salicylate on the LPS-induced synthesis of the pro-coagulant protein tissue factor (TF) and the pro-inflammatory protein tumor necrosis factor-alpha (TNF-alpha), as well as the prost

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::51e713ade9cef9f7585ad743007dd062
https://pubmed.ncbi.nlm.nih.gov/8972019

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Elevated albumin excretion rate is common among poorly controlled adolescent insulin dependent diabetics

Autor: H J, Bangstad, K F, Hanssen, P, Kierulf, S, Larsen, K, Dahl-Jørgensen, G B, Joø, O, Aagenaes

Publikováno v: Diabetes research (Edinburgh, Scotland). 6(1)

We studied urinary albumin excretion in 2 different groups of teenage diabetics, one with HbA1 less than 10% (n = 15) and another with HbA1 greater than 13% (n = 17). We found a highly significant difference between the 2 groups regarding the albumin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f8d96c3b06f3eaaa8350c195255984bc
https://pubmed.ncbi.nlm.nih.gov/3690956

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