Zobrazeno 1 - 10
of 32
pro vyhledávání: '"G A, Velikodvorskaia"'
Autor:
G A, Velikodvorskaia, L A, Chekanovskaia, N A, Lunina, O V, Sergienko, V G, Lunin, I A, Dvortsov, V V, Zverlov
Publikováno v:
Molekuliarnaia biologiia. 47(4)
The nucleotide sequence of a chromosome fragment of the thermophilic anaerobic bacterium Caldicellulosiruptor bescii (syn. Anaerocellum thermophilum) has been determined. The fragment contains four open reading frames with the second one of 749 aa en
Autor:
V G, Lunin, N E, Sharapova, T V, Tikhonova, N N, Poletaeva, Z M, Galushkina, E I, Aksenova, V I, Grabko, G A, Velikodvorskaia, N V, Lavrova, Iu V, Anan'ina
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (1)
Development of new technology allows different antigens of a necessary degree of cleanliness to be obtained. This development is a major problem of modern medical biotechnology. A promising approach to this problem includes use of the affinity domain
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (1)
The problem of search for and characterization of enzymes synthesized by non-cultivated microorganisms is presently being settled by creating metagenomic libraries. A 6000-clone library with the average size of its inserts amounting to 15 bp has been
Publikováno v:
Molekuliarnaia biologiia. 39(6)
The nucleotide sequence of a 4936 bp Thermoanaerobacter ethanolicus genomic DNA fragment containing the thermostable beta-galactosidase gene lacA and two incomplete open reading frames has been determined. The product of the first frame is highly hom
[Prospects for practical application of substrate-binding modules of glycosyl hydrolases (A review)]
Publikováno v:
Prikladnaia biokhimiia i mikrobiologiia. 40(5)
The properties of substrate-binding modules of glycosyl hydrolases have been reviewed. Variation of the properties of these modules makes them promising as components of chimeric proteins, which is a rapidly developing field of biotechnology. Example
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (1)
A search for phytase genes in 9 Bacillus strains from the collection of IMGAN was implemented. The growth optimum of strains IX-22, IX-12B, K17-2, K18, IMG I, IMG II, M4 and M8 was 50-60 degrees C; the optimal growth temperature for Bacillus sp. 790
Autor:
N A, Fokina, G A, Velikodvorskaia
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (2)
An anaerobic thermophilic bacterium Thermoanaerobacter ethanolicus 39E (Clostridium thermohydrosulfuricum 39E) gene library was constructed in E. coli. Recombinant plasmid (pUT50) containing the thermostable beta-galactosidase was isolated by direct
Publikováno v:
Biokhimiia (Moscow, Russia). 58(8)
The endoglucanase (EG5) has been isolated from the recombinant strain of E. coli TG1 carrying a plasmid with C. thermocellum F7 chromosomal DNA insertion. Using high performance ion-exchange chromatography and chromatofocusing, the enzyme was 98-fold
Publikováno v:
Antibiotiki. 20(7)
A new virus capable of replication on E. coli C was isolated from Penicillium chrysogenum. Six strains of P. chrysogenum differing in the cultural-morphological and biochemical properties and the titer of virus PBV5 were studied. The data provided a
Publikováno v:
Genetika. 21(6)
Bacteriophage Mu is characterized by a phenomenon similar to the transposition immunity of TnA: the frequency of transposition of Mu or mini-Mu into plasmids containing certain phage sequences is reduced by two orders of magnitude. In order to lend t