Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Gökhan Tolun"'
Autor:
Zhi-Qiang Xu, Slobodan Jergic, Allen T. Y. Lo, Alok C. Pradhan, Simon H. J. Brown, James C. Bouwer, Harshad Ghodke, Peter J. Lewis, Gökhan Tolun, Aaron J. Oakley, Nicholas E. Dixon
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Abstract Ring-shaped DNA sliding clamps are essential for DNA replication and genome maintenance. Clamps need to be opened and chaperoned onto DNA by clamp loader complexes (CLCs). Detailed understanding of the mechanisms by which CLCs open and place
Externí odkaz:
https://doaj.org/article/9e8880c9aa2c40dbb2212545d9c48737
Publikováno v:
Engineering Microbiology, Vol 4, Iss 1, Pp 100120- (2024)
Recombineering is an essential tool for molecular biologists, allowing for the facile and efficient manipulation of bacterial genomes directly in cells without the need for costly and laborious in vitro manipulations involving restriction enzymes. Th
Externí odkaz:
https://doaj.org/article/26db5ad3b59847bd925a78c8f3e21d7a
Autor:
Timothy P. Newing, Jodi L. Brewster, Lucy J. Fitschen, James C. Bouwer, Nikolas P. Johnston, Haibo Yu, Gökhan Tolun
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Redβ annealase catalyses single-strand annealing homologous DNA recombination. Here, the authors present a cryo-EM structure of a Redβ annealing intermediate bound to two complementary 27mer oligonucleotides.
Externí odkaz:
https://doaj.org/article/d8eae846edef420e98dca3a781c83034
Autor:
Timothy P. Newing, Aaron J. Oakley, Michael Miller, Catherine J. Dawson, Simon H. J. Brown, James C. Bouwer, Gökhan Tolun, Peter J. Lewis
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Gram-positive bacteria contain a transcription factor HelD that is able to remove and recycle stalled transcription complexes. Here the authors provide mechanistic insights into this process by determining the cryo-EM structures of the Bacillus subti
Externí odkaz:
https://doaj.org/article/5ba51ba3f90b4dc994ae7757d1617c0b
Autor:
Arnab Roy Chowdhury, Rahul Bakshi, Jianyang Wang, Gokben Yildirir, Beiyu Liu, Valeria Pappas-Brown, Gökhan Tolun, Jack D Griffith, Theresa A Shapiro, Robert E Jensen, Paul T Englund
Publikováno v:
PLoS Pathogens, Vol 6, Iss 12, p e1001226 (2010)
Introduced in the 1950s, ethidium bromide (EB) is still used as an anti-trypanosomal drug for African cattle although its mechanism of killing has been unclear and controversial. EB has long been known to cause loss of the mitochondrial genome, named
Externí odkaz:
https://doaj.org/article/fb8e2012b7964a0091c8eb39ae88ecf7
Autor:
Timothy P, Newing, Jodi L, Brewster, Lucy J, Fitschen, James C, Bouwer, Nikolas P, Johnston, Haibo, Yu, Gökhan, Tolun
Publikováno v:
Nature communications. 13(1)
The Redβ protein of the bacteriophage λ red recombination system is a model annealase which catalyzes single-strand annealing homologous DNA recombination. Here we present the structure of a helical oligomeric annealing intermediate of Redβ, consi
Autor:
Catherine J. Dawson, James C. Bouwer, Aaron J. Oakley, Timothy P. Newing, Michael Miller, Gökhan Tolun, Simon H. J. Brown, Peter J. Lewis
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications
Nature Communications
In bacteria, transcription complexes stalled on DNA represent a major source of roadblocks for the DNA replication machinery that must be removed in order to prevent damaging collisions. Gram-positive bacteria contain a transcription factor HelD that
Autor:
Timothy Newing, Jodi L. Brewster, Haibo Yu, Nikolas P. Johnston, Lucy J. Fitschen, Gökhan Tolun
The bacteriophage λ red recombination system catalyzes the single-strand annealing homologous DNA recombination reaction, in which Redβ annealase protein plays a critical role. Using cryogenic electron microscopy, we were able to determine a struct
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::671bdea2a39032f806e7c158e1a5d96d
https://doi.org/10.1101/2022.04.09.487726
https://doi.org/10.1101/2022.04.09.487726
Autor:
Gökhan Tolun, Jodi L. Brewster
Publikováno v:
Iubmb Life
DNA recombination, replication, and repair are intrinsically interconnected processes. From viruses to humans, they are ubiquitous and essential to all life on Earth. Single‐strand annealing homologous DNA recombination is a major mechanism for the
Autor:
Anna Louise Doss, Joshua A. Knapp, Nancy L. Craig, Gökhan Tolun, Thomas Laver, Hosam Ewis, Fred Dyda, Ad Bax, Alasdair C. Steven, Xianghong Li, Alexander Grishaev, Alison B. Hickman, Peter W. Atkinson
Publikováno v:
Cell. 158(2):353-367
Summary Hermes is a member of the hAT transposon superfamily that has active representatives, including McClintock's archetypal Ac mobile genetic element, in many eukaryotic species. The crystal structure of the Hermes transposase-DNA complex reveals