Zobrazeno 1 - 10
of 44
pro vyhledávání: '"Gérard Dubray"'
Autor:
Michel S. Zygmunt, Florence Mompart, Florence Berthelot-Hérault, Gérard Dubray, Marion Duchet-Suchaux
Publikováno v:
Veterinary Immunology and Immunopathology
Veterinary Immunology and Immunopathology, Elsevier, 2003, 96 (1-2), pp.43-52
Veterinary Immunology and Immunopathology, Elsevier, 2003, 96 (1-2), pp.43-52
Salmonella frequently causes human foodborne infections. Contaminated products from poultry infected with Salmonella enteritidis are mainly involved. This serovar is able to colonize the gastrointestinal tract and generally produces a chronic asympto
Publikováno v:
Journal of Medical Microbiology. 47:39-48
The antibody response specific to the 25-kDa major outer-membrane protein (Omp25) of Brucella melitensis expressed in Escherichia coli was assessed in BALB/c mice. Groups of mice were immunised and boosted either with sonicated E. coli carrying plasm
Autor:
Michel S. Zygmunt, Guy Bézard, Ana Paula Teixeira-Gomes, Axel Cloeckaert, Raul A. Bowden, Gérard Dubray
Publikováno v:
Electrophoresis. 18:1491-1497
In a previous report, proteins from Brucella melitensis were characterized by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and N-terminal microsequencing. In the present report, we have extended this study to the second etiologic age
Publikováno v:
Electrophoresis. 18:156-162
Two-dimensional (2-D) gel electrophoresis was used to map Brucella melitensis proteins. The 2-D proteins map of B. melitensis B115 revealed 595 silver-stained protein spots separated by both isoelectric point and molecular mass. Twenty-five proteins
Publikováno v:
Europe PubMed Central
The problem of differentiating sheep infected with Brucella melitensis from those vaccinated or exposed to cross-reaching organisms has not been resolved by conventional serological tests or through the use of the smooth lipopolysaccharide in primary
Publikováno v:
Veterinary Microbiology. 44:37-48
Distinction between Brucella melitensis infected and vaccinated sheep is needed to fully achieve ovine brucellosis eradication in several countries. For this purpose, we probed immunoblots of cytosoluble protein extract (CPE) of the rough (R) B. meli
Publikováno v:
Research in Microbiology. 146:227-236
Changes in Brucella cell envelope protein profiles were investigated with batch cultures of B. melitensis strain 16M in a 2-litre fermenter. Analysis of expression of outer membrane proteins (OMP) (apparent molecular masses of 10, 16.5, 19, 25-27, 31
Publikováno v:
Veterinary Microbiology
Veterinary Microbiology, Elsevier, 1994, 39, pp.33-46
Veterinary Microbiology, Elsevier, 1994, 39, pp.33-46
Sera from Brucella infected and B. melitensis Rev1 vaccinated sheep were analysed by immunoblotting using the cell envelope fraction (CEF) of B. melitensis B115. The CEF of B. melitensis B115 was analysed using a bank of monoclonal antibodies. The fr
Publikováno v:
Journal of Medical Microbiology. 39:403-407
The antibody response of cattle to the minor 89-kDa outer-membrane protein (OMP) of brucella was measured by indirect ELISA with the purified protein and compared with the antibody response to smooth lipopolysaccharide (S-LPS). Pre-incubating sera wi
Publikováno v:
Research in Microbiology. 144:475-484
We characterized 4 monoclonal antibodies (mAb) specific for rough lipopolysaccharide (R-LPS) of Brucella. mAb were selected by enzyme-linked immunosorbent assay (ELISA) on whole B. abortus 45/20 rough cells and R-LPS from B. melitensis B115 rough cel