In Situ-downstream Processing of Recombinant Histidine-tagged Proteins From Cultivations of Bacillus Megaterium

Autor: Gädke, Johannes

Purification of (recombinant) proteins for industrial and pharmaceutical use is accompanied by high costs and difficulties in the scale-up with the necessity of many purification steps. This thesis demonstrates the use of functionalized superparamagn

Integrated in situ-purification of recombinant proteins from Bacillus megaterium cultivation using SPION in stirred tank reactors

Autor: Gädke, Johannes, Thies, Jan-Wilhelm, Kleinfeldt, Lennart, Kalinin, Artem, Starke, Gereon, Lakowitz, Antonia, Biedendieck, Rebekka, Garnweitner, Georg, Dietzel, Andreas, Krull, Rainer

Publikováno v: In Biochemical Engineering Journal 15 October 2017 126:58-67

Spray-Dried Hierarchical Aggregates of Iron Oxide Nanoparticles and Their Functionalization for Downstream Processing in Biotechnology

Autor: Kleinfeldt, Lennart, Gädke, Johannes, Biedendieck, Rebekka, Krull, Rainer, Garnweitner, Georg

Publikováno v: ACS Omega, Vol 4, Iss 15, Pp 16300-16308 (2019)
ACS Omega
ACS Omega, 2019, 4, 15, 16300-16308--http://pubs.acs.org/journal/acsodf--https://www.ncbi.nlm.nih.gov/pmc/journals/3686/--http://www.bibliothek.uni-regensburg.de/ezeit/?2861993--2470-1343--2470-1343

In this work, the structuring of iron oxide nanoparticles via spray-drying (SD) of aqueous suspensions is investigated, leading to micrometer-sized aggregates with saturation magnetization comparable to that of the individual nanoparticles. Interesti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::8e8b3ade8574cf5aaab6936ae7124fc9
https://doaj.org/article/442144dccbff420cbca55fe10a970b50

Novel Dynamic Model to Predict the Glycosylation Pattern of Monoclonal Antibodies from Extracellular Cell Culture Conditions

Autor: Gädke Johannes, Moo-Young Murray, Budman M. Hector, Aghamohseni Hengameh, Scharer Jeno, Ohadi Kaveh, Legge L. Raymond

Publikováno v: IFAC Proceedings Volumes. 46:30-35

Glycosylation is a critical protein post-translational modification with a profound impact on the therapeutic properties of Mab and research indicates that it depends on extracellular culture conditions. A novel dynamic model was developed to relate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::782988b5c9a21159b1fecd942f163959
https://doi.org/10.3182/20131216-3-in-2044.00009

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