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Publikováno v:
Glia. 31(1)
The present study investigates the presence of vitamin D receptor (VDR) in cells of the rat oligodendrocyte (OL) lineage. VDR transcripts were detected by in situ hybridization in a fraction of rat OL in secondary cultures. The VDR protein was shown
Autor:
C, Jacquin-Becker, G, Labourdette
Publikováno v:
Glia. 20(2)
Aldose reductase (AR) is known to be responsible for many side effects of diabetes. In the present work, we studied the effects of various extracellular signals on the regulation of the expression of AR in astrocytes in culture, by determining its en
Publikováno v:
Journal of neuroscience research. 39(6)
Cultures of oligodendrocyte precursor cells can be grown from brain hemispheres of newborn rats. These cells, also called O-2A progenitor cells, can differentiate in vitro into oligodendrocytes or type 2 astrocytes. Basic FGF and PDGF are known to st
Publikováno v:
Journal of cellular physiology. 150(1)
Previous results (Fressinaud, C., Sarliève, L.L., and Labourdette, G. J. J. Cell. Physiol., 141:667-674, 1989b) have shown that cerebroside sulfotransferase (CST; EC 2.8.2.11) is enriched in pure rat oligodendrocyte (OL) cultures and that its activi
Publikováno v:
Journal of cellular physiology. 149(3)
Glutamine synthetase (GS, EC 6.3.1.2.) has long been considered as a protein specific for astrocytes in the brain, but recently GS immunoreactivity has been reported in oligodendrocytes both in mixed primary glial cell cultures and in vivo. We have i
Autor:
P, Rogue, G, Labourdette, A, Masmoudi, Y, Yoshida, F L, Huang, K P, Huang, J, Zwiller, G, Vincendon, A N, Malviya
Publikováno v:
The Journal of biological chemistry. 265(7)
Rat liver nuclei protein kinase C is identified as type II isozyme employing monospecific antibodies obtained against each three types of rat brain protein kinase C isozymes. (Yoshida, Y., Huang, F. L., Nakabayashi, H., and Huang, K-P. (1988) J. Biol
Publikováno v:
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie. 310(7)
We describe a method of partial purification of rat brain 3'-phosphoadenosine-5'-phosphosulfate galactocerebroside sulfotransferase (EC 2.8.2.11, CST). The first steps consist of a Triton X-100 extraction of the enzyme from delipidated microsomes and
Publikováno v:
Journal de physiologie. 82(4)
We purified to homogeneity two active factors, named astroglial growth factors (AGFs: AGF2 and AGF1), from bovine brain after two and three chromatographic steps, respectively. We found that AGFs have a strong affinity for heparin. Therefore, heparin
Publikováno v:
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie. 308(10)
The transforming growth factor beta (TGF beta) is a weak mitogen for rat oligodendrocytes grown in serum-free chemically defined medium. When these cells were treated by basic fibroblast growth factor (bFGF), which is the most potent known mitogen fo