Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Fusao Kimizuka"'
Publikováno v:
Nucleic Acids Research. 21:2309-2313
The genes from Moraxella bovis encoding the MboI restriction--modification system were cloned and expressed in Escherichia coli. Three open reading frames were found in the sequence containing the genes. These genes, which we named mboA, mboB, and mb
Autor:
Toshinaga Maeda, Fusao Kimizuka, Kiyotoshi Sekiguchi, Ikunoshin Kato, Kimikazu Hashino, Koiti Titani, Tomoko Shimojo
Publikováno v:
The Journal of Biochemistry. 112:547-551
A new artificial cell adhesive protein was engineered by grafting the Arg-Gly-Asp (RGD) sequence, the minimal recognition signal of fibronectin for interaction with integrins, to a calpastatin segment by in vitro mutagenesis. The mutagenized protein
Autor:
Fusao Kimizuka, Ikunoshin Kato, Harue Okuyama, Yukuo Ishizaki, Yoshihiro Matsumoto, Takashi Makabe, Jun Murata, Junya Yoneda, Ichiro Azuma, Ikuo Saiki
Publikováno v:
Japanese Journal of Cancer Research : Gann
We have investigated the effect of recombinant polypeptides with cell‐binding domain (C‐274) or with heparin‐binding domain (H‐271) and their fusion polypeptide (CH‐271) on liver metastasis of murine lymphoid tumor. The polypeptides contain
Autor:
Hiroyuki Hanzawa, Takashi Murachi, Masakazu Hatanaka, Kiyozo Asada, Yoji Arata, Fusao Kimizuka, Tadao Asano, Masatoshi Maki, Ikunoshin Kato, Takashi Uemori, Tomoko Shimojo
Publikováno v:
Biochemical and Biophysical Research Communications. 166:1485-1493
Expression plasmids were constructed from the cDNA of human calpastatin to examine the contribution to the inhibition of calpain of highly conserved sequences in each of four repetitive domains. A series of deletion derivatives of domain 1 proteins,
Autor:
Fusao Kimizuka, Ikunoshin Kato, Tomoko Shimojo, Yoichi Ohdate, Yuki Taguchi, Yasutoshi Kawase
Publikováno v:
FEBS Letters. 298:126-128
An efficient expression system was constructed for C-EGF, a fusion protein made of a fragment of the cell-binding domain of human fibronectin (FN) bound with epidermal growth factor (EGF), C-EGF was produced in Escherichia coli HB101 cells carrying t
Autor:
Akihiko Kita, Fusao Kimizuka, Shu Matsushita, Katsutoshi Mise, Michiko Miyahara, Yasuo Kudo, Toshio Shimada
Publikováno v:
Biologicalpharmaceutical bulletin. 19(11)
Five restriction endonucleases (ENases) and one ENase were found in a screen of 196 strains of Plesiomonas shigelloides and 147 strains of Aeromonas species. Plesiomonas and Aeromonas species are classified as Vibrionaceae, identified as food-poisoni
Publikováno v:
Journal of biochemistry. 119(4)
The binding of fibronectin to fibronectin receptor was studied using a recombinant 31-kDa cell-binding domain fragment of fibronectin (C279), which consisted of three type III repeats (III8-III9-III10). Fibronectin receptor in several cell lysates wa
Publikováno v:
Gene. 157(1-2)
We isolated and characterized from a Streptomyces species a new class-II restriction endonuclease, which recognizes the palindromic heptanucleotide sequence 5′-G G↓G W C C C 3′-C C C W G↑G G (where W = A or T) and cleaves double-stranded DNA
Autor:
Yu Igarashi, Yukuo Ishizaki, Ichiro Azuma, Hideo Kobayashi, Hideki Fujii, Fusao Kimizuka, Ikunosin Kato, Junya Yoneda, Ikuo Saiki
Publikováno v:
Experimental cell research. 217(1)
Cellular responses to fibronectin (FN) are likely to have a complex molecular basis involving the interactions between multiple functional domains of FN and specific cell surface molecules. We have utilized several types of recombinant FN fragments a
Autor:
Raymond L. Rodriguez, Hiroyuki Ito, Akira Ohbayashi, Hirokazu Matsui, Yuko Ohashi, Alan B. Caplan, Mamoru Honma, Atsuhiko Shinmyo, Fusao Kimizuka
Publikováno v:
Plant Cell Reports. 13
PCR with oligonucleotide primers that corresponded to two highly homologous regions, in terms of amino acid sequence, of plant peroxidases was used to amplify a specific DNA fragment from a mixture of rice (Oryza sativa L.) cDNAs. We then screened a