Zobrazeno 1 - 10
of 121
pro vyhledávání: '"Fu-Kuen Lin"'
Publikováno v:
Protein Expression and Purification. 8:215-226
This report describes bacterial expression, isolation, and characterization of human tissue kallikrein recombinantly produced in Escherichia coli. Successful production of enzymatically active recombinant human kallikrein requires the following proce
Publikováno v:
Journal of Cardiovascular Pharmacology. 15:S7-S16
Tissue kallikreins are a group of closely related serine proteinases that are represented by multigene families in mice and rats. The existence of similar, large, kallikrein-like gene families in other mammalian species is currently a matter of dispu
Autor:
Migliaccio, Anna Rita1 (AUTHOR) amigliaccio@altius.org
Publikováno v:
Biomolecules (2218-273X). Apr2024, Vol. 14 Issue 4, p408. 29p.
Publikováno v:
Protein expression and purification. 8(2)
We report here the expression of recombinant human prokallikrein and kallikrein in engineered Chinese hamster ovary cells transfected with a human genomic gene encoding preprokallikrein. At high expression levels, recombinant prokallikrein, an inacti
Autor:
Chuan Chu Chou, Hsieng Sen Lu, Julie Chao, Fu-Kuen Lin, William Bacheller, Kenneth Chen, Lee Chao, Charles J. Herrera, Chi-Hwei Lin, Ted Jones
Publikováno v:
Biochimica et biophysica acta. 1173(3)
Cynomolgus monkey renal kallikrein cDNA and genomic human tissue kallikrein gene were cloned. The monkey gene encodes a 257 amino acid (aa) preprokallikrein and exhibits 95% and 92% homology to the human at nucleotide (nt) and aa level, respectively.
Autor:
Simon H. Chang, Fu-kuen Lin
Publikováno v:
Mechanisms of Ageing and Development. 11:383-392
The change of the specific activity of S-adenosylmethionine: tRNA methyltransferase in cultures of human diploid fibroblasts at different passages has been measured and compared with that in the same type of cells derived from donors of different age
Publikováno v:
Blood. 71:104-109
Erythroid differentiation is mediated by several interacting factors which include the glycoprotein hormone erythropoietin (Epo), interleukin-3 (IL-3) in the mouse, and erythroid-potentiating activity (EPA) in humans. Each of these factors binds to s
Publikováno v:
Blood. 72:970-977
The contention that erythropoietin (Epo) affects platelet production was investigated in the rat with recombinant human Epo (rHuEpo). In normal rats, Epo caused a dose-dependent increase in both reticulocyte and platelet numbers, the reticulocyte res
Autor:
Por-Hsiung Lai, Joan C. Egrie, Chi-Hwei Lin, Sidney Vaughn Suggs, R. Smalling, Kenneth Chen, Fu-Kuen Lin, J K Browne, G. M. Fox, M. Castro
Publikováno v:
Gene. 44:201-209
The erythropoietin (Epo) gene from Cynomolgus monkeys has been isolated from a kidney cDNA library using mixed 20-mer oligodeoxynucleotide probes. The gene encodes a 168 amino acid (aa) mature protein with a calculated Mr of 18,490 and a presumptive
Autor:
Sunoo, Brenda Paik
Publikováno v:
Personnel Journal. Feb96, Vol. 75 Issue 2, p38. 6p. 3 Color Photographs, 1 Graph.