Zobrazeno 1 - 10
of 146
pro vyhledávání: '"Fritz Paltauf"'
Publikováno v:
Protein Science. 8:215-221
The lipases from Rhizopus and Rhizomucor are members of the family of Mucorales lipases. Although they display high sequence homology, their stereoselectivity toward triradylglycerols (sn-2 substituted triacylglycerols) varies. Four different trirady
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1734:215-219
N-Acylethanolamines (NAEs) and N-acylphosphatidylethanolamines (NAPEs) are trace constituents of vertebrate cells and tissues and much is known about their metabolism and possible function in animals. Here we report for the first time the identificat
Autor:
Andreas Potthoff, Peter Stadler, Friedrich Spener, Lutz Haalck, Sabine Irmer, Johannes Bucher, Andrea Kovac, Fritz Paltauf
Publikováno v:
European Journal of Lipid Science and Technology. 102:173-180
In a model elaborated earlier to understand and predict the stereopreference ofRhizopus oryzae lipase (ROL) catalyzed hydrolysis of triradylglycerols, we identified the degree of flexibility of the C1′-X′ bond (X = O for ether, N for amide, C for
Publikováno v:
European Journal of Lipid Science and Technology. 102:61-77
Lipases exhibit specific catalytic properties that make them attractive to biotechnological applications. Most important are the broad substrate specificity and the regio- and stereoselectivity of lipases. Despite mechanistic and structural similarit
Autor:
Lutz Haalck, Friedrich Spener, H. Lindner, Fritz Paltauf, M. Graupner, Albin Hermetter, Otto Glatter
Publikováno v:
Biophysical Journal. 77:493-504
We have studied the intrinsic tryptophan fluorescence of the lipases from Chromobacterium viscosum (CVL), Pseudomonas species (PSL), and Rhizopus oryzae (ROL) in aqueous buffer, zwitterionic detergent micelles, and isopropanol-water mixtures. It was
Publikováno v:
European Journal of Biochemistry. 262:63-69
Fluorescent triacylglycerol analogs were synthesized as covalent inhibitors of lipase activity. The respective 1(3), 2-O-dialkylglycero-3(1)-alkyl-phosphonic acid p-nitrophenyl esters contain a fluorescent pyrenealkyl chain and a long-chain alkyl res
Publikováno v:
Colloids and Surfaces A: Physicochemical and Engineering Aspects. 139:189-197
Solvent-free bilayer lipid membranes (BLM) and unilamellar liposomes composed of mixed chain phosphatidylcholines, containing 1–3 double bonds in the hydrocarbon chains in position sn-2, have been studied by various physico-chemical techniques. The
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 3:127-130
A new type of fluorogenic alkyldiacyl glycerols was synthesized and used as fluorogenic substrates for the analysis of lipase activities and stereoselectivities. These compounds contain pyrene or perylene as a fluorophore and the trinitrophenyl-amino
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1304:229-244
1,2(2,3)-Diradylglycero O-(p-nitrophenyl) n-hexylphosphonates were synthesized, with the diradylglycerol moiety being di-O-octylglycerol, 1-O-hexadecyl-2-O-pyrenedecanylglycerol, or 1-O-octyl-2-oleoyl-glycerol, and tested for their ability to inactiv
Publikováno v:
FEBS Letters. 393:236-240
A membrane-bound phospholipase D (PLD) from Saccharomyces cerevisiae was solubilized from mitochondrial and plasma membranes and partially purified. The enzyme has an apparent molecular weight of approximately 60 kDa, is strictly Ca(2+)-dependent and