Zobrazeno 1 - 10
of 239
pro vyhledávání: '"Friedrich C Simmel"'
Autor:
Florian Katzmeier, Lukas Aufinger, Aurore Dupin, Jorge Quintero, Matthias Lenz, Ludwig Bauer, Sven Klumpe, Dawafuti Sherpa, Benedikt Dürr, Maximilian Honemann, Igor Styazhkin, Friedrich C Simmel, Michael Heymann
Publikováno v:
PLoS ONE, Vol 14, Iss 12, p e0220091 (2019)
Point-of-care testing (POCT) in low-resource settings requires tools that can operate independently of typical laboratory infrastructure. Due to its favorable signal-to-background ratio, a wide variety of biomedical tests utilize fluorescence as a re
Externí odkaz:
https://doaj.org/article/298bfe3ccbbc47b69cb02d1334f6b578
Publikováno v:
PLoS ONE, Vol 13, Iss 9, p e0198058 (2018)
CRISPR interference (CRISPRi) using dCas9-sgRNA is a powerful tool for the exploration and manipulation of gene functions. Here we quantify the reversible switching of a central process of the bacterial cell cycle by CRISPRi and an antisense RNA mech
Externí odkaz:
https://doaj.org/article/c87416c5f18b420e8c13c527664e07ab
Autor:
Andreas Walbrun, Tianhe Wang, Michael Matthies, Petr Šulc, Friedrich C. Simmel, Matthias Rief
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)
Abstract Toehold-mediated strand displacement (TMSD) is extensively utilized in dynamic DNA nanotechnology and for a wide range of DNA or RNA-based reaction circuits. Investigation of TMSD kinetics typically relies on bulk fluorescence measurements p
Externí odkaz:
https://doaj.org/article/d72d93fd266f4b93bc887d75ef8537ac
Autor:
Tiago Ramalho, Andrea Meyer, Andrea Mückl, Korbinian Kapsner, Ulrich Gerland, Friedrich C Simmel
Publikováno v:
PLoS ONE, Vol 11, Iss 1, p e0145829 (2016)
Monitoring gene expression dynamics on the single cell level provides important information on cellular heterogeneity and stochasticity, and potentially allows for more accurate quantitation of gene expression processes. We here study bacterial sende
Externí odkaz:
https://doaj.org/article/97ae112679c04543ba96c2735e834a95
Autor:
Zhijin Tian, Dandan Shao, Linlin Tang, Zhen Li, Qian Chen, Yongxiu Song, Tao Li, Friedrich C. Simmel, Jie Song
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-11 (2024)
Abstract Cell-free protein expression (CFE) systems have emerged as a critical platform for synthetic biology research. The vectors for protein expression in CFE systems mainly rely on double-stranded DNA and single-stranded RNA for transcription and
Externí odkaz:
https://doaj.org/article/94299ba348ab40d190b5079e4b354b67
Autor:
Friedrich C. Simmel
Publikováno v:
RNA Biology, Vol 20, Iss 1, Pp 154-163 (2023)
Nucleic acid strand displacement reactions involve the competition of two or more DNA or RNA strands of similar sequence for binding to a complementary strand, and facilitate the isothermal replacement of an incumbent strand by an invader. The proces
Externí odkaz:
https://doaj.org/article/63a35ed62e8741aa8ceea749406aa806
Autor:
Linlin Tang, Zhijin Tian, Jin Cheng, Yijing Zhang, Yongxiu Song, Yan Liu, Jinghao Wang, Pengfei Zhang, Yonggang Ke, Friedrich C. Simmel, Jie Song
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)
Abstract Synthetic gene networks in mammalian cells are currently limited to either protein-based transcription factors or RNA-based regulators. Here, we demonstrate a regulatory approach based on circular single-stranded DNA (Css DNA), which can be
Externí odkaz:
https://doaj.org/article/8c442d47cfe44ab4a50d5e718540b83c
Autor:
Florian Katzmeier, Friedrich C. Simmel
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-11 (2023)
Abstract Second-order electrokinetic flow around colloidal particles caused by concentration polarization electro-osmosis (CPEO) can result in a phoretic motion of asymmetric particle dimers in a homogeneous AC electrical field, which we refer to as
Externí odkaz:
https://doaj.org/article/8a60f4242f5c4b468bd29e2ff4b59297
Autor:
Matthias Vogt, Martin Langecker, Matthias Gouder, Enzo Kopperger, Florian Rothfischer, Friedrich C. Simmel, Jonathan List
Publikováno v:
Nature Physics. 19:741-751
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::cb718a4617bf0a6bdf451cc91f87e0b4
https://doi.org/10.1038/s41567-023-01938-3
https://doi.org/10.1038/s41567-023-01938-3
Publikováno v:
Journal of the American Chemical Society. 145:634-644
Toehold-mediated strand displacement (TMSD) has been used extensively for molecular sensing and computing in DNA-based molecular circuits. As these circuits grow in complexity, sequence similarity between components can lead to cross-talk causing lea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f0b6c81bc6284bdc504a7363d467b33
https://doi.org/10.1021/jacs.2c11208
https://doi.org/10.1021/jacs.2c11208