Zobrazeno 1 - 10
of 49
pro vyhledávání: '"Friedemann Hesse"'
Publikováno v:
Re:GEN Open, Vol 4, Iss 1, Pp 21-32 (2024)
Introduction: In the past decade, microRNAs (miRNAs), which are small, noncoding RNAs of ?22 nucleotides length, have gained attention as novel engineering tools for biopharmaceutical cell line development. As miRNAs provide the ability to fine-tune
Externí odkaz:
https://doaj.org/article/5a19ab4869a748beab4d00d04faf56e0
Autor:
Patrick Schlossbauer, Lukas Naumann, Florian Klingler, Madina Burkhart, René Handrick, Kathrin Korff, Christian Neusüß, Kerstin Otte, Friedemann Hesse
Publikováno v:
Engineering in Life Sciences, Vol 24, Iss 6, Pp n/a-n/a (2024)
Abstract Cell engineering strategies typically rely on energy‐consuming overexpression of genes or radical gene‐knock out. Both strategies are not particularly convenient for the generation of slightly modulated phenotypes, as needed in biosimila
Externí odkaz:
https://doaj.org/article/8dcdbbb84fc94f5d93f3c2f682b96283
Autor:
Karen Schwab, Friedemann Hesse
Publikováno v:
Bioengineering, Vol 4, Iss 3, p 65 (2017)
Multi-wavelength fluorescence spectroscopy was evaluated in this work as tool for real-time monitoring of antibody aggregation in CHO fed-batch cultivations via partial least square (PLS) modeling. Therefore, we used the extrinsic fluorescence dyes 1
Externí odkaz:
https://doaj.org/article/a2d31369c18946dd81ba7b8167223568
Publikováno v:
Bioengineering, Vol 3, Iss 4, p 32 (2016)
The glycosyltransferase HisDapGalNAcT2 is the key protein of the Escherichia coli (E. coli) SHuffle® T7 cell factory which was genetically engineered to allow glycosylation of a protein substrate in vivo. The specific activity of the glycosyltransfe
Externí odkaz:
https://doaj.org/article/6eab7610aaf54a92b7ab52f55d548bab
Autor:
Lukas Naumann, Patrick Schlossbauer, Florian Klingler, Friedemann Hesse, Kerstin Otte, Christian Neusüß
Publikováno v:
Journal of Separation Science. 45:2034-2044
The analysis of monoclonal antibodies glycosylation is a crucial quality control attribute of biopharmaceutical drugs. High throughput screening approaches for antibody glycoform analysis are required in various stages of process optimization. Here,
Publikováno v:
The Analyst. 144:6334-6341
Antibody aggregates may occur as undesirable by-products during the manufacturing process of biopharmaceutical proteins since parameters such as pH, temperature, ionic strength, protein concentration, oxygen, and shear forces can lead to aggregate fo
Publikováno v:
Biotechnology and Bioengineering. 115:1173-1185
Protein aggregation of monoclonal antibodies (mAbs) is a common phenomenon associated with the production of these biopharmaceuticals. These aggregates can lead to adverse side effects in patients upon administration, thus expensive downstream proces
Autor:
Verena V. Emmerling, Stefan Kochanek, Simon Fischer, Karlheinz Holzmann, René Handrick, Fabian Stiefel, Friedemann Hesse, Markus Hörer, Kerstin Otte
Publikováno v:
Biotechnology and Bioengineering. 113:830-841
Cell engineering and bioprocess optimizations such as low temperature cultivation represent powerful tools to improve cellular performance and product yields of mammalian production cells. Besides monoclonal antibodies (mABs), novel biotherapeutic fo
Autor:
Friedemann Hesse, Melanie Geiss, Joerg Zimmermann, Andreas Wagner, René Handrick, Kerstin Otte, Simon Fischer, Sven Mathias, Martin Domnowski, Albert Jesuran Paul, Franziska Schandock
Publikováno v:
Biotechnology and Bioengineering. 112:2142-2153
Histone deacetylase (HDAC) inhibitors have been exploited for years to improve recombinant protein expression in mammalian production cells. However, global HDAC inhibition is associated with negative effects on various cellular processes. microRNAs
Autor:
Friedemann Hesse, Nicole Borth, René Handrick, Johannes Grillari, Matthias Hackl, Simon Fischer, Kerstin Otte, Fabian Stiefel
Publikováno v:
Pharmaceutical Bioprocessing. 3:227-247
Chinese hamster ovary cells are among the biotechnologically most relevant production systems for biopharmaceuticals. With the availability of the genomic sequences of Chinese hamster, rational systems biotechnology-driven approaches to optimize this