Structural basis of organic cation transporter-3 inhibition

Autor: Basavraj Khanppnavar, Julian Maier, Freja Herborg, Ralph Gradisch, Erika Lazzarin, Dino Luethi, Jae-Won Yang, Chao Qi, Marion Holy, Kathrin Jäntsch, Oliver Kudlacek, Klaus Schicker, Thomas Werge, Ulrik Gether, Thomas Stockner, Volodymyr M. Korkhov, Harald H. Sitte

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)

The current work reports the structure of the human organic cation transporter 3 (OCT3 / SLC22A3) and provides the structural basis of its inhibition by two specific inhibitors, decynium-22 and corticosterone.

Externí odkaz: https://doaj.org/article/c390514bb74345508edbb1e105eb04af

Multimodal detection of dopamine by sniffer cells expressing genetically encoded fluorescent sensors

Autor: Carmen Klein Herenbrink, Jonatan Fullerton Støier, William Dalseg Reith, Abeer Dagra, Miguel Alejandro Cuadrado Gregorek, Reto B. Cola, Tommaso Patriarchi, Yulong Li, Lin Tian, Ulrik Gether, Freja Herborg

Publikováno v: Communications Biology, Vol 5, Iss 1, Pp 1-9 (2022)

Dopamine sensor cell lines expressing fluorescent reporters are developed. This versatile tool can record endogenous dopamine release from cultured neurons and striatal slices, determine dopamine tissue content, and detect dopamine uptake and efflux.

Externí odkaz: https://doaj.org/article/f96e4b28cf164fb29c3362861b49966d

Increased vulnerability of nigral dopamine neurons after expansion of their axonal arborization size through D2 dopamine receptor conditional knockout.

Autor: Nicolas Giguère, Benoît Delignat-Lavaud, Freja Herborg, Aurore Voisin, Yuan Li, Vincent Jacquemet, Madhu Anand-Srivastava, Ulrik Gether, Bruno Giros, Louis-Éric Trudeau

Publikováno v: PLoS Genetics, Vol 15, Iss 8, p e1008352 (2019)

Parkinson's disease (PD) is a neurodegenerative disorder characterized by the loss of dopamine (DA) neurons in the substantia nigra pars compacta (SNc). Rare genetic mutations in genes such as Parkin, Pink1, DJ-1, α-synuclein, LRRK2 and GBA are foun

Externí odkaz: https://doaj.org/article/f0618051b02541699df966c28c410d7e

Relative enrichment – a density-based colocalization measure for single-molecule localization microscopy

Autor: Aske L. Ejdrup, Matthew D. Lycas, Niels Lorenzen, Ainoa Konomi, Freja Herborg, Kenneth L. Madsen, Ulrik Gether

Dual-color single-molecule localization microscopy (SMLM) provides unprecedented possibilities for detailed studies of colocalization of different molecular species in a cell. However, the informational richness of the data is not fully exploited by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::768bb6c4588ae69bd83f4b2a10f9a52a
https://doi.org/10.1101/2022.02.08.479535

Nanoscopic dopamine transporter distribution and conformation are inversely regulated by excitatory drive and D2 autoreceptor activity

Autor: Matthew D. Lycas, Aske L. Ejdrup, Andreas T. Sørensen, Nicolai O. Haahr, Søren H. Jørgensen, Daryl A. Guthrie, Jonatan F. Støier, Christian Werner, Amy Hauck Newman, Markus Sauer, Freja Herborg, Ulrik Gether

Publikováno v: Lycas, M D, Ejdrup, A L, Sørensen, A T, Haahr, N O, Jørgensen, S H, Guthrie, D A, Støier, J F, Werner, C, Newman, A H, Sauer, M, Herborg, F & Gether, U 2022, ' Nanoscopic dopamine transporter distribution and conformation are inversely regulated by excitatory drive and D2 autoreceptor activity ', Cell Reports, vol. 40, no. 13, 111431 . https://doi.org/10.1016/j.celrep.2022.111431

The nanoscopic organization and regulation of individual molecular components in presynaptic varicosities of neurons releasing modulatory volume neurotransmitters like dopamine (DA) remain largely elusive. Here we show, by application of several supe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8f93a5627fa7fbc1d538b2bf8f7ed9f
https://doi.org/10.1016/j.celrep.2022.111431