Zobrazeno 1 - 10
of 4 193
pro vyhledávání: '"Free protein"'
Publikováno v:
Beilstein Journal of Organic Chemistry, Vol 20, Iss 1, Pp 2242-2253 (2024)
Biocatalysis has established itself as a successful tool in organic synthesis. A particularly fast technique for screening enzymes is the in vitro expression or cell-free protein synthesis (CFPS). The system is based on the transcription and translat
Externí odkaz:
https://doaj.org/article/dddafadf2cd6499e9ad34cb0097baba5
Publikováno v:
BioMedInformatics, Vol 4, Iss 3, Pp 2022-2042 (2024)
Cell-free protein synthesis (CFPS) has emerged as a powerful tool for protein production, with applications ranging from basic research to biotechnology and pharmaceutical development. However, enhancing the efficiency of CFPS systems remains a cruci
Externí odkaz:
https://doaj.org/article/dcb7fa844eb44cedb2fd955436e155ed
Autor:
Jeffrey L. Schloßhauer, Lena Tholen, Alexander Körner, Stefan Kubick, Sofia Chatzopoulou, Anja Hönow, Anne Zemella
Publikováno v:
Synthetic and Systems Biotechnology, Vol 9, Iss 3, Pp 416-424 (2024)
Chinese hamster ovary (CHO) cells are crucial in biopharmaceutical production due to their scalability and capacity for human-like post-translational modifications. However, toxic proteins and membrane proteins are often difficult-to-express in livin
Externí odkaz:
https://doaj.org/article/135f28cf17b64878a5997cacbe96703b
Autor:
Ana L. J. L. Ribeiro, Patricia Pérez-Arnaiz, Mercedes Sánchez-Costa, Lara Pérez, Marcos Almendros, Liisa van Vliet, Fabrice Gielen, Jesmine Lim, Simon Charnock, Florian Hollfelder, J. Eduardo González-Pastor, José Berenguer, Aurelio Hidalgo
Publikováno v:
Microbial Cell Factories, Vol 23, Iss 1, Pp 1-14 (2024)
Abstract Background In vitro expression involves the utilization of the cellular transcription and translation machinery in an acellular context to produce one or more proteins of interest and has found widespread application in synthetic biology and
Externí odkaz:
https://doaj.org/article/fabf2155b3e74a7ca7d5bcf1d40d2296
Autor:
Kodai Machida, Rin Tanaka, Seraya Miki, Shotaro Noseda, Mayumi Yuasa-Sunagawa, Hiroaki Imataka
Publikováno v:
BioTechniques, Vol 76, Iss 4, Pp 161-168 (2024)
Programmed-1 ribosomal frameshifting (-1 PRF) is a translational mechanism adopted by some viruses, including SARS-CoV-2. To find a compound that can inhibit -1 PRF in SARS-CoV-2, we set up a high-throughput screening system using a HeLa cell extract
Externí odkaz:
https://doaj.org/article/7aeabe3ef8ea485eb824b825ecaed003
Publikováno v:
eLife, Vol 13 (2024)
Experimental detection of residues critical for protein–protein interactions (PPI) is a time-consuming, costly, and labor-intensive process. Hence, high-throughput PPI-hot spot prediction methods have been developed, but they have been validated us
Externí odkaz:
https://doaj.org/article/02fbed149f19420a840b80310265b0b7
Autor:
Sarfaraz K. Niazi, Matthias Magoola
Publikováno v:
Biologics, Vol 3, Iss 4, Pp 355-379 (2023)
Recombinant technology has been around for nearly three quarters of a century and has revolutionized protein therapy. However, the cost of developing recombinant therapeutic proteins and the manufacturing infrastructure keeps their cost unaffordable
Externí odkaz:
https://doaj.org/article/f346e5a73b1c4bf795faa2ac6bbd8f8a
Akademický článek
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Autor:
Anne Zemella, Kerstin Beer, Franziska Ramm, Dana Wenzel, Ariane Düx, Kevin Merkel, Sebastien Calvignac-Spencer, Daniel Stern, Martin B. Dorner, Brigitte G. Dorner, Navena Widulin, Thomas Schnalke, Cornelia Walter, Anne Wolbert, Bernhard G. Schmid, Annette Mankertz, Sabine Santibanez
Publikováno v:
International Journal of Medical Microbiology, Vol 314, Iss , Pp 151607- (2024)
Measles is a highly contagious airborne viral disease. It can lead to serious complications and death and is preventable by vaccination. The live-attenuated measles vaccine (LAMV) derived from a measles virus (MV) isolated in 1954 has been in use glo
Externí odkaz:
https://doaj.org/article/a8672cb3b85e473f936d7711a30fe5e2
Publikováno v:
Frontiers in Natural Products, Vol 3 (2024)
Peptide natural products have a wide range of useful applications as pesticides, veterinary agents, pharmaceuticals, and bioproducts. To discover new natural products, manipulate them for analog generation, and to harness the potential of these bioac
Externí odkaz:
https://doaj.org/article/2389086540cc49908e6a3cca38c6a540