Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Frederick S Gimble"'
Publikováno v:
PLoS ONE, Vol 9, Iss 2, p e88840 (2014)
Genetic modification of a chromosomal locus to replace an existing dysfunctional allele with a corrected sequence can be accomplished through targeted gene correction using the cell's homologous recombination (HR) machinery. Gene targeting is stimula
Externí odkaz:
https://doaj.org/article/3ce394158ba24f668fcf0cb80d0a81d1
Autor:
Frederick S. Gimble, Kok Zhi Lee, Michael A. Mechikoff, Arren Liu, Kevin Fitzgerald, Paula Pandolfi, Kevin V. Solomon, Archana Kikla
Publikováno v:
Nucleic Acids Research
Prokaryotic Argonautes (pAgos) have been proposed as more flexible tools for gene-editing as they do not require sequence motifs adjacent to their targets for function, unlike popular CRISPR/Cas systems. One promising pAgo candidate, from the halophi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46cad1013c5f880eac3fc9d371999f3d
http://europepmc.org/articles/PMC8464042
http://europepmc.org/articles/PMC8464042
Autor:
Rasika R. Nawimanage, Ziyan Yuan, Mackenzie Casares, Rakesh Joshi, Jeremy R. Lohman, Frederick S. Gimble
Publikováno v:
Journal of molecular biology. 434(9)
The LAGLIDADG family of homing endonucleases (LHEs) bind to and cleave their DNA recognition sequences with high specificity. Much of our understanding for how these proteins evolve their specificities has come from studying LHE homologues. To gain i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8d8207a2e3d39e94b3c0be8ffb9850c
https://pubmed.ncbi.nlm.nih.gov/35317996
https://pubmed.ncbi.nlm.nih.gov/35317996
Autor:
Kok Zhi Lee, Michael A. Mechikoff, Archana Kikla, Paula Pandolfi, Arren Liu, Kevin Fitzgerald, Frederick S. Gimble, Kevin V. Solomon
Prokaryotic Argonautes (pAgos) have been proposed as more flexible tools for gene-editing as they do not require sequence motifs adjacent to their targets for function, unlike popular CRISPR/Cas systems. One promising pAgo candidate, from the halophi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::01d6b73e0ef237b50a41dabb81a5f0b6
https://doi.org/10.1101/597237
https://doi.org/10.1101/597237
Publikováno v:
Journal of Molecular Biology. 382:188-202
The number of strand-specific nicking endonucleases that are currently available for laboratory procedures and applications in vivo is limited, and none is sufficiently specific to nick single target sites within complex genomes. The extreme target s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebd8b82cb00203b2014bfed50fed0536
https://doi.org/10.1016/j.jmb.2008.07.010
https://doi.org/10.1016/j.jmb.2008.07.010
Autor:
Frederick S. Gimble
Publikováno v:
Gene Therapy and Regulation. :33-50
Gene targeting to selected chromosomal loci is greatly stimulated when free DNA ends are created that initiate double-strand break repair. Gene therapy reagents can be developed by engineering DNA endonucleases that cleave genomes at desired target s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::030c32fbd3b63078848d4a0d1fefe5bd
https://doi.org/10.1142/s1568558607000034
https://doi.org/10.1142/s1568558607000034
Publikováno v:
Nucleic Acids Research. 32:3947-3956
Homing endonuclease genes (HEGs) are mobile DNA elements that are thought to confer no benefit to their host. They encode site-specific DNA endonucleases that perpetuate the element within a species population by homing and disseminate it between spe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b5702d259fea338fffd4e958eb8b959
https://doi.org/10.1093/nar/gkh734
https://doi.org/10.1093/nar/gkh734
Publikováno v:
Journal of Molecular Biology. 334:993-1008
The PI-SceI protein from Saccharomyces cerevisiae is a member of the LAGLIDADG family of homing endonucleases that have been used in genomic engineering. To assess the flexibility of the PI-SceI-binding interaction and to make progress towards the di
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fe572d7bd5bca7061debfcfcb0c5f13
https://doi.org/10.1016/j.jmb.2003.10.013
https://doi.org/10.1016/j.jmb.2003.10.013
Publikováno v:
Nature Structural Biology. 9:764-770
The first X-ray structures of an intein-DNA complex, that of the two-domain homing endonuclease PI-SceI bound to its 36-base pair DNA substrate, have been determined in the presence and absence of Ca(2+). The DNA shows an asymmetric bending pattern,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd3ad4ae51a475dc43de89bb46b0b954
https://doi.org/10.1038/nsb840
https://doi.org/10.1038/nsb840
Autor:
Frederick S. Gimble, Karen L. Posey
Publikováno v:
Biochemistry. 41:2184-2190
Target sites for homing endonucleases occur infrequently in complex genomes. As a consequence, these enzymes can be used in mammalian systems to introduce double-strand breaks at recognition sites inserted within defined loci to study DNA repair by h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0422cff22e4a9605a1f72a73e9357c3
https://doi.org/10.1021/bi015944v
https://doi.org/10.1021/bi015944v