The Get1/2 insertase forms a channel to mediate the insertion of tail-anchored proteins into the ER

Autor: Paul Heo, Jacob A. Culver, Jennifer Miao, Frederic Pincet, Malaiyalam Mariappan

Publikováno v: Cell Reports, Vol 42, Iss 1, Pp 111921- (2023)

Summary: Tail-anchored (TA) proteins contain a single C-terminal transmembrane domain (TMD) that is captured by the cytosolic Get3 in yeast (TRC40 in humans). Get3 delivers TA proteins to the Get1/2 complex for insertion into the endoplasmic reticulu

Externí odkaz: https://doaj.org/article/3140225396d546c5a27daedc9d467434

Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules

Autor: Gamal Rayan, Jean-Erik Guet, Nicolas Taulier, Frederic Pincet, Wladimir Urbach

Publikováno v: Sensors, Vol 10, Iss 6, Pp 5927-5948 (2010)

This review examines some recent applications of fluorescence recovery after photobleaching (FRAP) to biopolymers, while mainly focusing on membrane protein studies. Initially, we discuss the lateral diffusion of membrane proteins, as measured by FRA

Externí odkaz: https://doaj.org/article/c5212a1f345448a487e196ff45b5a487

Common intermediates and kinetics, but different energetics, in the assembly of SNARE proteins

Autor: Sylvain Zorman, Aleksander A Rebane, Lu Ma, Guangcan Yang, Matthew A Molski, Jeff Coleman, Frederic Pincet, James E Rothman, Yongli Zhang

Publikováno v: eLife, Vol 3 (2014)

Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are evolutionarily conserved machines that couple their folding/assembly to membrane fusion. However, it is unclear how these processes are regulated and function. To det

Externí odkaz: https://doaj.org/article/0013692539554ac5ab8cd80a5e10e695

Actuating tension-loaded DNA clamps drives membrane tubulation

Autor: Longfei Liu, Qiancheng Xiong, Chun Xie, Frederic Pincet, Chenxiang Lin

Publikováno v: Science advances. 8(41)

Membrane dynamics in living organisms can arise from proteins adhering to, assembling on, and exerting force on cell membranes. Programmable synthetic materials, such as self-assembled DNA nanostructures, offer the capability to drive membrane remode

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::031f32e2c0dbf6481a4b72d1df469c5c
https://pubmed.ncbi.nlm.nih.gov/36223466

Freezing and piercing of in vitro asymmetric plasma membrane by α-synuclein

Autor: Paul Heo, Frederic Pincet

Publikováno v: Communications Biology, Vol 3, Iss 1, Pp 1-8 (2020)
Communications Biology
Communications Biology, Nature Publishing Group, 2020, 3 (1), ⟨10.1038/s42003-020-0883-7⟩

Synucleinopathies are neurological diseases that are characterized by the accumulation of aggregates of a cytosolic protein, α-synuclein, at the plasma membrane. Even though the pathological role of the protein is established, the mechanism by which

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f535f369eea9a3e5c140bfa3fc22b1d
http://link.springer.com/article/10.1038/s42003-020-0883-7