Assessment of genetic and epigenetic changes during cell culture ageing and relations with somaclonal variation in Coffea arabica

Autor: Roberto Bobadilla Landey, Benoît Bertrand, Jamel Aribi, Philippe Lashermes, Romain Guyot, Eveline Dechamp, Frederic Georget, Hervé Etienne, Juan Carlos Herrera, Alberto Cenci

Publikováno v: Plant Cell, Tissue and Organ Culture
Plant Cell, Tissue and Organ Culture, 2015, 122 (3), pp.517-531. ⟨10.1007/s11240-015-0772-9⟩
Plant Cell, Tissue and Organ Culture, Springer Verlag, 2015, 122 (3), pp.517-531. ⟨10.1007/s11240-015-0772-9⟩

International audience; Long-term cell cultures were used in coffee to study the cytological, genetic and epigenetic changes occurring during cell culture ageing. The objective was toidentify the mechanisms associated with somaclonal variation (SV).

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c7707b5a0cafd240c1c30f45d641a11
https://doi.org/10.1007/s11240-015-0772-9

A single-step method for RNA isolation from tropical crops in the field

Autor: Frederic Georget, Benoît Bertrand, Hervé Etienne, Claudine Campa, Jean-Christophe Breitler

Publikováno v: Scientific Reports
Scientific Reports, Nature Publishing Group, 2016, 6 (1), pp.38368. ⟨10.1038/srep38368⟩
Scientific Reports, 2016, 6 (1), pp.38368. ⟨10.1038/srep38368⟩

The RNAzol RT reagent was used to provide pure RNA from human cells. We develop a protocol using RNAzol RT reagent to extract pure RNA from plants tissues and demonstrate that this RNA extraction method works not only at room temperature but also at

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cf4eff6240dd8310bf0bb06f85fafc5
https://doi.org/10.1038/srep38368

Creation and dissemination of Arabica coffee varieties: What varietal innovations?

Autor: Benoît Bertrand, Frederic Georget, Christophe Montagnon, Pierre Charmetant, Hervé Etienne

Publikováno v: Cahiers Agricultures. 21:77-88

Plus de 80 % de la production de cafe arabica provient d’Amerique latine alors que l’espece est originaire d’Afrique. La base genetique du cafeier Arabica introduite en Amerique latine au XIX e siecle se limite a deux populations peu differente

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fdc8ba11a5021263aabb11b987e8fbdf
https://doi.org/10.1684/agr.2012.0547

High genetic and epigenetic stability in Coffea arabica plants derived from embryogenic suspensions and secondary embryogenesis as revealed by AFLP, MSAP and the phenotypic variation rate

Autor: Benoît Bertrand, June Simpson, Sylvain Santoni, Frederic Georget, Juan Carlos Herrera, Eveline Dechamp, Hervé Etienne, Gloria Camayo, Alberto Cenci, Philippe Lashermes, Roberto Bobadilla Landey

Publikováno v: PLoS ONE
PLoS ONE, Public Library of Science, 2013, 8 (2), ⟨10.1371/journal.pone.0056372⟩
PloS One
Plos One 2 (8), . (2013)
PLoS ONE, Vol 8, Iss 2, p e56372 (2013)

Embryogenic suspensions that involve extensive cell division are risky in respect to genome and epigenome instability. Elevated frequencies of somaclonal variation in embryogenic suspension-derived plants were reported in many species, including coff

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b07df26c95e0f54e57ff872add2a2659
https://hal.archives-ouvertes.fr/hal-01268002

A comparative analysis of the development and quality of nursery plants derived from somatic embryogenesis and from seedlings for large-scale propagation of coffee (Coffea arabica L.)

Autor: Benoît Bertrand, Dominique Barry-Etienne, Hervé Etienne, Frederic Georget, Andrea Menéndez-Yuffá

Publikováno v: Plant Cell, Tissue and Organ Culture

Plants of Coffea arabica L. derived via somatic embryogenesis, namely, somaclones, were evaluated with C. arabica seedlings grown in the nursery. At the time of their transfer to the nursery, somaclones of C. arabica cvs. Caturra and Costa Rica 95 (C

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73c574bd3b675ae6807b14c95a6889ce
http://agritrop.cirad.fr/556398/