Contribution of amino acids in the active site of dipeptidyl peptidase 4 to the catalytic action of the enzyme.

Autor: Kathrin Gnoth, Joachim Wolfgang Bär, Fred Rosche, Jens-Ulrich Rahfeld, Hans-Ulrich Demuth

Publikováno v: PLoS ONE, Vol 19, Iss 4, p e0289239 (2024)

Dipeptidyl peptidase 4 (DP4)/CD26 regulates the biological function of various peptide hormones by releasing dipeptides from their N-terminus. The enzyme is a prominent target for the treatment of type-2 diabetes and various DP4 inhibitors have been

Externí odkaz: https://doaj.org/article/b643d60db2f543459ffef241fa9b6f9e

Simple Purification of Nicotiana benthamiana-Produced Recombinant Colicins: High-Yield Recovery of Purified Proteins with Minimum Alkaloid Content Supports the Suitability of the Host for Manufacturing Food Additives

Autor: Anett Stephan, Anatoli Giritch, Yuri Gleba, Fred Rosche, Simone Hahn-Löbmann, Mirko Buchholz

Publikováno v: International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 19; Issue 1; Pages: 95
International Journal of Molecular Sciences, Vol 19, Iss 1, p 95 (2017)

Colicins are natural non-antibiotic bacterial proteins with a narrow spectrum but an extremely high antibacterial activity. These proteins are promising food additives for the control of major pathogenic Shiga toxin-producing E. coli serovars in meat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::421beee7db6db5403e5f89416cbcdc13
https://pubmed.ncbi.nlm.nih.gov/29286298

Heterologous Expression and Characterization of Human Glutaminyl Cyclase: Evidence for a Disulfide Bond with Importance for Catalytic Activity

Autor: Hans-Ulrich Demuth, Stephan Schilling, Claus Wasternack, Susanne Manhart, Fred Rosche, Torsten Hoffmann

Publikováno v: Biochemistry. 41:10849-10857

Glutaminyl cyclase (QC, EC 2.3.2.5) catalyzes the formation of pyroglutamate residues from glutamine at the N-terminus of peptides and proteins. In the current study, human QC was functionally expressed in the secretory pathway of Pichia pastoris, yi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f395e1b7334d7dcb86e3f53dec2ce6ff
https://doi.org/10.1021/bi0260381

Immunoprecipitation and liquid chromatographic–mass spectrometric determination of the peptide glucose-dependent insulinotropic polypeptides GIP1-42 and GIP3-42 from human plasma samples

Autor: Hans-Ulrich Demuth, Torsten Hoffmann, Fred Rosche, Raik Wolf

Publikováno v: Journal of Chromatography A. 926:21-27

The gastrointestinal peptide glucose-dependent insulinotropic polypeptide (GIP1-42) is one of the incretin hormones regulating glucose-induced insulin secretion from the endocrine pancreas. GIP1-42 is a substrate of the circulating enzyme dipeptidyl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8f584d46171f61682bfc996315b10142
https://doi.org/10.1016/s0021-9673(01)00942-6

The Use of MALDI-TOF Mass Spectrometry In Quantification of the Stability of Prolyl Endopeptidase Inhibitors

Autor: Hans-Uirich Demuth, Jorn Schmidt, Michael Wermann, Fred Rosche

Publikováno v: Protein & Peptide Letters. 3:385-392

Abstract: Z-Phe-Pro-(isonicotinic acid methylester) methylketone is a highly potent inhibitor (Ki*= 18 nM) of prolyl endopeptidase from Flavobacterium meningosepticum. It acts as a slow-binding inhibitor and decomposes to compounds still possessing i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b21c38ba4672ad517770a52f14f189bc
https://doi.org/10.2174/092986650306221101113916