Zobrazeno 1 - 10
of 83
pro vyhledávání: '"Fred P, Abramson"'
Publikováno v:
Proceedings of the National Academy of Sciences. 101:8858-8863
A remote labeling method has been developed to determine 18 O kinetic isotope effects (KIEs) in Ras-catalyzed GTP hydrolysis. Substrate mixtures consist of 13 C-depleted GTP and [ 18 O, 13 C]GTP that contains 18 O at phosphoryl positions of mechanist
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d00ee1f6f31d9fa3bd43b8f58fff2ab7
https://doi.org/10.1073/pnas.0401675101
https://doi.org/10.1073/pnas.0401675101
Publikováno v:
American Journal of Kidney Diseases. 42:561-566
Background: Dialyzers reprocessed with chlorine-based solutions have been associated with increases in ultrafiltration coefficient and middle-molecule removal. Increased pore size has been hypothesized as the mechanism for the latter phenomenon. Dial
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a4f66d9eb29f546c7f171814a1e70c0
https://doi.org/10.1016/s0272-6386(03)00789-3
https://doi.org/10.1016/s0272-6386(03)00789-3
Publikováno v:
Journal of Biochemical and Biophysical Methods. 56:141-152
Size-exclusion chromatography (SEC) is a separation technique with a relatively low resolving power, compared to those usually utilized in proteomics. Therefore, it is often overlooked in experimental protocols, when the main goal is resolving comple
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20812d2833bb34b1522da061eedeaf52
https://doi.org/10.1016/s0165-022x(03)00055-1
https://doi.org/10.1016/s0165-022x(03)00055-1
Autor:
Fred P. Abramson
Publikováno v:
Seminars in Perinatology. 25:133-138
Although there is a long history of stable isotopes use in drug metabolism research, it is appropriate to evaluate them in pregnancy drug studies in which safety takes highest priority. It is well established through a number of human and animal expe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e298653f16dcb6914c54ae363837d551
https://doi.org/10.1053/sper.2001.24568
https://doi.org/10.1053/sper.2001.24568
Publikováno v:
Rapid Communications in Mass Spectrometry. 15:602-607
This paper describes the use of chemical reaction interface mass spectrometry (CRIMS) combined with liquid chromatography for the detection of trace level sulfur-containing impurities in pharmaceutical materials. A mixture of sulfur- and non-sulfur-c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1e135360b15336cb1a4f7fdb1807f825
https://doi.org/10.1002/rcm.257
https://doi.org/10.1002/rcm.257
Autor:
Fred P. Abramson, Paolo Lecchi
Publikováno v:
Analytical Chemistry. 71:2951-2955
Size exclusion chromatography (SEC) has been coupled to chemical reaction interface mass spectrometry (CRIMS) for the analysis of biopolymers. This innovative combination allows the analysis of biopolymers with no limitation on the molecular weight a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdb8c40a0271872baae4a2c3c482f367
https://doi.org/10.1021/ac981098r
https://doi.org/10.1021/ac981098r
Autor:
Fred P. Abramson, Paolo Lecchi
Publikováno v:
Journal of Chromatography A. 828:509-513
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f4ccf00c3dbc9dac7d63164ef166b2d
https://doi.org/10.1016/s0021-9673(98)00818-8
https://doi.org/10.1016/s0021-9673(98)00818-8
Autor:
Blaire L. Osborn, Fred P. Abramson
Publikováno v:
Biopharmaceutics & Drug Disposition. 19:439-444
We present a novel method for performing pharmacokinetic and metabolism studies on macromolecules that offers advantages over the existing techniques of radiolabeling, immunoassay or bioassays. Our strategy uses macromolecules with stable isotopes un
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1b17fc17be4ab8f10ff059e3ffbc4226
https://doi.org/10.1002/(sici)1099-081x(199810)19:7<439::aid-bdd124>3.0.co
https://doi.org/10.1002/(sici)1099-081x(199810)19:7<439::aid-bdd124>3.0.co
Autor:
Fred P. Abramson, Pei Chen
Publikováno v:
Analytical Chemistry. 70:1664-1669
We have devised and evaluated a stable-isotopic method for measuring DNA synthesis rates. The probe is [1-13C]-glycine that is incorporated into purines via de novo biosynthesis. The human hepatoma cell line HEP G2 was grown in medium containing [1-1
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d35ccdb3bbd6494aecbeb3b0cfaf16a
https://doi.org/10.1021/ac971294i
https://doi.org/10.1021/ac971294i
Autor:
Fred P. Abramson, Blaire L. Osborn
Publikováno v:
Journal of Labelled Compounds and Radiopharmaceuticals. 39:935-953
In order to implement a new strategy for determining the kinetics and metabolism of therapeutic proteins, we had to produce milligram quantities of a uniformly stable isotope labelled species. To produce the labelled protein, the rat pituitary tumor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dbff85e4e367a0654b6107ff9db4d601
https://doi.org/10.1002/(sici)1099-1344(199711)39:11<935::aid-jlcr37>3.0.co
https://doi.org/10.1002/(sici)1099-1344(199711)39:11<935::aid-jlcr37>3.0.co