Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Fred A.M. Asselbergs"'
Autor:
Fred A.M. Asselbergs, Sylvie Rival
Publikováno v:
BioTechniques, Vol 20, Iss 4, Pp 558-562 (1996)
Externí odkaz:
https://doaj.org/article/fe4fde552d0e4bcf9e639ad8fe342b81
Autor:
Garret M. Hampton, Jian Zhu, Marija Zeremski, Richard Cai, Dalia Cohen, Shaowen Wang, Denise Fischer, Yan Yan-Neale, Fred A.M. Asselbergs
Publikováno v:
Oncogene. 22:6204-6213
The dynamic balance between histone acetylation and deacetylation plays a significant role in the regulation of gene transcription. Much of our current understanding of this transcriptional control comes from the use of HDAC inhibitors such as trapox
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::329469d69bcd4b7fe64272c1335ff335
https://doi.org/10.1038/sj.onc.1206653
https://doi.org/10.1038/sj.onc.1206653
Autor:
Fred A.M. Asselbergs, Roland Widmer
Publikováno v:
Analytical Biochemistry. 318:221-229
Apoptosis could be measured in mammalian cells by measuring the degradation of the small cytoplasmic human RNA Y1 (hY1) by real-time quantitative fluorescent reverse transcriptase polymerase chain reaction (RT-PCR). In FAS-antibody-treated Jurkat T c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8f55233c01461f0d3062f8056c82cf8
https://doi.org/10.1016/s0003-2697(03)00218-5
https://doi.org/10.1016/s0003-2697(03)00218-5
Autor:
Michael O. Zang-Gandor, Christine Gandor, Peter J. Flor, Marcello Marangi, Fred A.M. Asselbergs
Publikováno v:
Biotechnology and Bioengineering. 65:523-528
SSF3 is a CHO cell line adapted for growth in protein-free medium. It grows in suspension unless serum-derived attachment factors such as vitronectin are added to the medium. Serum-independent cell lines, which adhere to the substrate after induction
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fe623fdb15f09010f0239c94caa8d1cb
https://doi.org/10.1002/(sici)1097-0290(19991205)65:5<523::aid-bit4>3.0.co
https://doi.org/10.1002/(sici)1097-0290(19991205)65:5<523::aid-bit4>3.0.co
Autor:
Mcmaster Gary Kent, Rainer Ortmann, Fred A.M. Asselbergs, Frank Buxton, Bastian Hengerer, Rita Grossenbacher, Esther Sutter, Roland Widmer
Publikováno v:
Nucleic Acids Research. 26:1826-1833
Two yeast artificial chromosomes containing the entire human nerve growth factor gene were isolated and mapped. By homologous recombination a luciferase gene was precisely engineered into the coding portion of the NGF gene and a neomycin selection ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc9859d46b67541edf5cc2f1eccdacc8
https://doi.org/10.1093/nar/26.7.1826
https://doi.org/10.1093/nar/26.7.1826
Autor:
Roland Widmer, Fred A.M. Asselbergs
Publikováno v:
Journal of Biotechnology. 43:133-138
The folA gene, the chromosomal dhfr gene of Escherichia coli, was engineered for expression in mammalian cells. In contrast to plasmid-derived bacterial dhfr genes previously used as selection markers in mammalian cells, the folA gene product is inhi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9050a0cb989318d9fa56431503795cd7
https://doi.org/10.1016/0168-1656(95)00131-3
https://doi.org/10.1016/0168-1656(95)00131-3
Publikováno v:
Journal of Biotechnology. 42:221-233
Development of a CHO cell-based production system for the hybrid plasminogen activator K2tu-PA is described. Using the major immediate-early promoter of mouse cytomegalovirus (MCMV) transient and stable expression levels were 3-10-fold higher than th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::615d32bba99ebe44a02aa08f3222c723
https://doi.org/10.1016/0168-1656(95)00082-2
https://doi.org/10.1016/0168-1656(95)00082-2
Autor:
Fred A.M. Asselbergs, Armin Fiechter, Helmut Trautmann, Jakob Reiser, Michael Zang, Christine Gandor, Ferruccio Messi, Christian Leist
Publikováno v:
Nature Biotechnology. 13:389-392
The growth-factor prototrophic Chinese hamster ovary (CHO) SSF3 cell line was previously adapted for growth in serum-free media. Here we present a newly designed medium which allows these cells to grow in the absence of any exogenously added growth f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f55ce7056b45edca3fa4bf6faba4788b
https://doi.org/10.1038/nbt0495-389
https://doi.org/10.1038/nbt0495-389
Publikováno v:
Journal of Biotechnology. 32:191-202
A process was developed to produce recombinant human renin for X-ray analysis and enzyme inhibition studies. An expression vector containing a human prorenin cDNA and expressing a mouse dihydrofolate reductase selection marker was transfected into dh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01943c385553aa68eab29f9da253014e
https://doi.org/10.1016/0168-1656(94)90182-1
https://doi.org/10.1016/0168-1656(94)90182-1
Publikováno v:
Blood Coagulation & Fibrinolysis. 4:27-33
uK2t-PA is a hybrid plasminogen activator in which the epidermal growth factor-like domain of the urokinase-type plasminogen activator precedes the kringle 2 and catalytic domains of tissue-type plasminogen activator. The molecules are expressed in C
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e97b9d48df227dc29e2cca2a85cb3946
https://doi.org/10.1097/00001721-199302000-00005
https://doi.org/10.1097/00001721-199302000-00005