Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Franz Dubois"'
Publikováno v:
European Journal of Biochemistry. 268:1392-1399
The cholesterol complexing agent methyl-cyclodextrin (MCD) provides an efficient mean for the removal of cholesterol from biological membranes. In order to study the effects of this agent on the lysosomal membrane in situ, we treated HepG2 cells with
Autor:
David E. Sleat, Peter Lobel, Li Lin, Istvan Sohar, Robert Wattiaux, Ming Sing Hsu, Franz Dubois, Michel Jadot, John E. Pintar, Simone Wattiaux-De Coninck
Publikováno v:
The Journal of Biological Chemistry. 274(30):21104-21113
The intracellular transport of soluble lysosomal enzymes relies on the post-translational modification of N-linked oligosaccharides to generate mannose 6-phosphate (Man 6-P) residues. In most cell types the Man 6-P signal is rapidly removed after tar
Publikováno v:
European Journal of Biochemistry. 249:862-869
Most lysosomal hydrolases are soluble enzymes. Lamp-II (lysosome-associated membrane protein-II) is a major constituent of the lysosomal membrane. We studied the aggregation of a series of lysosomal molecules. The aggregation-sensitive lysosomal mark
Publikováno v:
Biochemical and Biophysical Research Communications. 227:448-454
Plasmid 35S DNA, naked or associated with different cationic lipid preparations was injected to rats. Subcellular distribution of radioactivity in the liver one hour after injection, was established by centrifugation methods. Results show that at tha
Autor:
Anick Claessens, Simone Wattiaux-De Coninck, Michel Jadot, Franz Dubois, Robert Wattiaux, Francis Mainferme
Publikováno v:
Biochemical and Biophysical Research Communications. 223(2):353-359
Lamp II (for lysosomal associated membrane protein II) is an integral type I glycoprotein. It consists of a very large and heavily glycosylated luminal domain, a single transmembrane segment, and a short cytoplasmic tail. We show that in highly purif
Publikováno v:
Biochemical and Biophysical Research Communications. 220(3):569-574
To study the transfer of phagocytosed components from phagosomes to lysosomes, we have investigated phagocytosis by rat liver of killed Staphylococcus aureus labelled with (125)I tyramine cellobiose. Lysosomes were identified by injecting the animals
Publikováno v:
Biochemical and Biophysical Research Communications. 213(1):81-87
We have investigated by using centrifugation methods, the uptake and the intracellular fate of 35S DNA by rat liver and the effect on these processes of N-(1-(2,3-dioleoxyloxy)propyl)-N,N,N,-trimethylammonium-methyl-sul fate(DOTAP, Boehringer, Mannhe
Publikováno v:
Biochemical Pharmacology. 44:1243-1248
When a light mitochondrial fraction (L fraction) of rat liver is incubated in the presence of an oxygen free radical generating system (xanthine-xanthine oxidase), the free activity of N-acetylglucosaminidase (NAGase) increases as a result of the det
Publikováno v:
Biochemical and Biophysical Research Communications. 158:313-318
We have investigated the endocytosis by rat liver of asialofetuin coupled to [ 125 I] tyramine cellobiose: [ 125 I] TCASF. Subcellular distribution of radioactive compounds was established after differential and isopyenic centrifugation and by analys
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 471:421-435
When maintained in the vicinity of the lower transition temperature of their membrane lipids, rat-liver mitochondria undergo lysis as shown by the release of malate dehydrogenase, (an enzyme located within the mitochondrial matrix), in the surroundin