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pro vyhledávání: '"Franz Dachs"'
Autor:
Erwin Schauenstein, G. Kollenz, Renate Horejsi, Waltraud Steinschifter, Franz Dachs, H. M. Tillian, K. Schauenstein
Publikováno v:
Journal of Biochemical and Biophysical Methods. 34:227-236
Immunoglobulin G (IgG) of many species contains 'labile' disulfide bonds (SS*), which within 24 h undergo a disulfide exchange with dithionitrobenzoic acid (NBSSBN). Aims of the present study were to detect directly this type of SS* groups by means o
Autor:
Astrid Leitsberger, Carola Hirschmann, Monika Reiter, Karin Maninger, M. Weblacher, Waltraud Steinschifter, Konrad Schauenstein, Franz Dachs, P. Felsner, Helene Horejsi, Erwin Schauenstein
Publikováno v:
IUBMB Life. 40:433-446
A reactive disulfide bond (SS)* was detected and characterized in IgG of humans, rats and mice by virtue of disulfide interchange with dithionitrobenzoate. (SS)* was found exclusively in human IgG1 and rat IgG2b. In human IgG1 (SS)* was identified as
Publikováno v:
Journal of Immunological Methods. 50:51-56
The IgG fraction was isolated from freshly taken blood serum of healthy persons (male and female) by column chromatography on QAE-Sephadex. After 30 min incubation with DTNB (5,5′-dithio-(2,2′-dinitro)-benzoate) the average photometrically determ