An anchored experimental design and meta-analysis approach to address batch effects in large-scale metabolomics

Autor: Amanda O. Shaver, Brianna M. Garcia, Goncalo J. Gouveia, Alison M. Morse, Zihao Liu, Carter K. Asef, Ricardo M. Borges, Franklin E. Leach, Erik C. Andersen, I. Jonathan Amster, Facundo M. Fernández, Arthur S. Edison, Lauren M. McIntyre

Publikováno v: Frontiers in Molecular Biosciences, Vol 9 (2022)

Untargeted metabolomics studies are unbiased but identifying the same feature across studies is complicated by environmental variation, batch effects, and instrument variability. Ideally, several studies that assay the same set of metabolic features

Externí odkaz: https://doaj.org/article/9d83b8419e124151806b3aaea729b8cd

A Taguchi Design of Experiments Approach for Untargeted Metabolomics Sample Preparation Optimization

Autor: Brianna M. Garcia, Goncalo J. Gouveia, Amanda O. Shaver, Ricardo M. Borges, I. Jonathan Amster, Arthur S. Edison, Franklin E. Leach III

Metabolomics commonly uses analytical techniques such as nuclear magnetic resonance (NMR) and liquid chromatography coupled to mass spectrometry (LC-MS) to quantify and identify metabolites associated with biological variation. Metabolome coverage fr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::17ba456b7d5d56d2041589c01939e043
https://doi.org/10.26434/chemrxiv-2022-znvj7

Microflow liquid chromatography – multi-emitter nanoelectrospray mass spectrometry of oligonucleotides

Autor: Guilherme J. Guimaraes, Franklin E Leach, Michael G. Bartlett

Publikováno v: Journal of Chromatography A. 1696:463976

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::704d04fff3778dbe393f67e1af8cdd6a
https://doi.org/10.1016/j.chroma.2023.463976

The specificity of the malarial VAR2CSA protein for chondroitin sulfate depends on 4-O-sulfation and ligand accessibility

Autor: Patience Sanderson, Thomas Mandel Clausen, Ana L. Saldanha, Yang Mao, Alejandro Gomez Toledo, Ali Salanti, Franklin E. Leach rd, Francesco Gatto, I. Jonathan Amster, Swati Choudhary, Charlotte B Spliid, Ismail Gögenur, Jeffrey D. Esko, Thor G. Theander, Tobias Gustavsson, Rasmus Peuliche Vogelsang

Publikováno v: Spliid, C B, Toledo, A G, Sanderson, P, Mao, Y, Gatto, F, Gustavsson, T, Choudhary, S, Saldanha, A L, Vogelsang, R P, Gögenur, I, Theander, T G, Leach, F E, Amster, I J, Esko, J D, Salanti, A & Clausen, T M 2021, ' The specificity of the malarial VAR2CSA protein for chondroitin sulfate depends on 4-O-sulfation and ligand accessibility ', The Journal of Biological Chemistry, vol. 297, no. 6, 101391 . https://doi.org/10.1016/j.jbc.2021.101391
J Biol Chem

Placental malaria infection is mediated by the binding of the malarial VAR2CSA protein to the placental glycosaminoglycan, chondroitin sulfate. Recombinant sub-fragments of VAR2CSA (rVAR2) have also been shown to bind specifically and with high affin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9d01fe78602eda5c3b23bdd5e22350a
https://curis.ku.dk/ws/files/287629835/1_s2.0_S0021925821011972_main.pdf

Sequencing the Dermatan Sulfate Chain of Decorin

Autor: Fuming Zhang, I. Jonathan Amster, Toshihiko Toida, Hong Zhang, Jiana Duan, Yanlei Yu, Franklin E. Leach, Kyohei Higashi, Robert J. Linhardt

Publikováno v: Journal of the American Chemical Society. 139:16986-16995

Glycomics represents one of the last frontiers and most challenging in omic analysis. Glycosylation occurs in the endoplasmic reticulum and the Golgi organelle and its control is neither well-understood nor predictable based on proteomic or genomic a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab470edde8886bc0ebe1b05881be0ccb
https://doi.org/10.1021/jacs.7b10164