Zobrazeno 1 - 10
of 39
pro vyhledávání: '"Frank C. Greene"'
Publikováno v:
Plant Science. 102:69-80
Numerous genes encoding agronomically important traits of crop plants are expressed in endosperm tissue. To facilitate the analysis of promoter regions from these genes, a transient assay was established using protoplasts from a maize (Zea mays L.) e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fabb114bb3d8fb994f5f8bb678a98650
https://doi.org/10.1016/0168-9452(94)90022-1
https://doi.org/10.1016/0168-9452(94)90022-1
Publikováno v:
Gene. 116:119-127
The development of efficient methods for production and purification of plant seed storage proteins in heterologous microbial hosts would facilitate structure-function studies of these proteins. This report describes such methods applied to the produ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::608ab00d07d4beb64e44d01facef0ef6
https://doi.org/10.1016/0378-1119(92)90507-l
https://doi.org/10.1016/0378-1119(92)90507-l
Publikováno v:
Plant Physiology. 98:433-441
The high molecular weight glutenin subunits are considered one of the most important components of wheat (Triticum aestivum) gluten, but their structure and interactions with other gluten proteins are still unknown. Understanding the role of these pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::202d9d805a7247957572bd990d396d41
https://doi.org/10.1104/pp.98.2.433
https://doi.org/10.1104/pp.98.2.433
Publikováno v:
Improvement of Cereal Quality by Genetic Engineering ISBN: 9781461360377
Progress is reported on the goal of our laboratory to use biotechnological approaches to improve wheat quality. The wheat gene family we have chosen for our initial efforts is that of the high-molecular-weight (HMW) glutenins. These polypeptides play
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c81b4d012054b2b1abde5fb898acab83
https://doi.org/10.1007/978-1-4615-2441-0_11
https://doi.org/10.1007/978-1-4615-2441-0_11
Autor:
Thomas W. Okita, Frank C. Greene
Publikováno v:
Plant Physiology. 69:834-839
A total RNA extract was prepared from developing wheat seeds using guanidine-HCl to eliminate endogenous RNase activity. The RNA preparation, substantially free of protein, carbohydrate and DNA, was chromatographed on either a poly uridylic acid-agar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::669d9175b76eac7848c03665cb2ae11f
https://doi.org/10.1104/pp.69.4.834
https://doi.org/10.1104/pp.69.4.834
Publikováno v:
Theoretical and Applied Genetics. 75:117-126
The nucleotide and deduced amino acid sequences of a high molecular weight glutenin subunit gene derived from chromosome 1B of bread wheat (Triticum aestivum L.) are reported. The encoded protein corresponds to the y-type subunit 1B9. Comparison of t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8d15da0adcf7a3b9eb9dab5ea5d97c42
https://doi.org/10.1007/bf00249151
https://doi.org/10.1007/bf00249151
Publikováno v:
Journal of the Science of Food and Agriculture. 32:773-780
In extracts of developing seeds of wheat (Triticum aestivum L., cultivars Scout 66, Cheyenne, Nugaines and INIA 66R) examined by polyacrylamide gel electrophoresis, gliadin proteins appeared at 12-15 days post-anthesis with mobilities covering nearly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5cab7b77d20e9571c34a0bea94c20079
https://doi.org/10.1002/jsfa.2740320805
https://doi.org/10.1002/jsfa.2740320805
Publikováno v:
Plant Physiology. 75:393-396
The amount of pyruvate orthophosphate dikinase (PPDK) (EC 2.7.9.1) protein in wheat ( Triticum aestivum L. var Cheyenne) grains was determined at different stages of development by the protein blot method. The variation in PPDK protein with time in d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4ff7c6b0bd1980ac00637b287e01cefc
https://doi.org/10.1104/pp.75.2.393
https://doi.org/10.1104/pp.75.2.393
Autor:
Frank C. Greene
Publikováno v:
Journal of Protein Chemistry. 3:167-180
A family of fluorescent probes, consisting of 2-p-toluidinylnapththalene-6-sulfonate (TNS) and neutral and cationic sulfonamido derivatives has been utilized to study the influence of electrostatic forces in protein-amphiphile interactions. 2-p-Tolui
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0d8258b502244b96aa6a8c15c6089a7e
https://doi.org/10.1007/bf01040498
https://doi.org/10.1007/bf01040498
Autor:
Frank C. Greene
Publikováno v:
Plant Physiology. 68:778-783
Free and membrane-associated polysomes were isolated in approximately equal amounts from endosperm of wheat kernels harvested 20 days after anthesis. The presence of heparin in the homogenizing buffer minimized polysome degradation. Ribonucleic acid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::851720dbaee301632bafbcd75b06d940
https://doi.org/10.1104/pp.68.3.778
https://doi.org/10.1104/pp.68.3.778