Proteomics of the dentate gyrus reveals semantic dementia specific molecular pathology

Autor: Merel O. Mol, Suzanne S. M. Miedema, Shamiram Melhem, Ka Wan Li, Frank Koopmans, Harro Seelaar, Kurt Gottmann, Volkmar Lessmann, Netherlands Brain Bank, August B. Smit, John C. van Swieten, Jeroen G. J. van Rooij

Publikováno v: Acta Neuropathologica Communications, Vol 10, Iss 1, Pp 1-14 (2022)

Abstract Semantic dementia (SD) is a clinical subtype of frontotemporal dementia consistent with the neuropathological diagnosis frontotemporal lobar degeneration (FTLD) TDP type C, with characteristic round TDP-43 protein inclusions in the dentate g

Externí odkaz: https://doaj.org/article/8c2d7e6c8ed449b1816eb6adb95fcab9

Brain Region Differences in α1- and α5-Subunit-Containing GABAA Receptor Proteomes Revealed with Affinity Purification and Blue Native PAGE Proteomics

Autor: Miao Chen, Frank Koopmans, Miguel A. Gonzalez-Lozano, August B. Smit, Ka Wan Li

Publikováno v: Cells, Vol 13, Iss 1, p 14 (2023)

GABAA receptors are the major inhibitory receptors in the brain. They are hetero-pentamers with a composition of predominantly two α, two β, and one γ or δ subunit. Of the six α subunit genes, the α5 subunit displays a limited spatial expressio

Externí odkaz: https://doaj.org/article/d7f9b4d52a7645c69f1d564e94edeec5

Suspension TRAPping Filter (sTRAP) Sample Preparation for Quantitative Proteomics in the Low µg Input Range Using a Plasmid DNA Micro-Spin Column: Analysis of the Hippocampus from the 5xFAD Alzheimer’s Disease Mouse Model

Autor: Evangelia Thanou, Frank Koopmans, Débora Pita-Illobre, Remco V. Klaassen, Berna Özer, Ioannis Charalampopoulos, August B. Smit, Ka Wan Li

Publikováno v: Cells, Vol 12, Iss 9, p 1242 (2023)

Suspension TRAPping filter (sTRAP) is an attractive sample preparation method for proteomics studies. The sTRAP protocol uses 5% SDS that maximizes protein solubilization. Proteins are trapped on a borosilicate glass membrane filter, where SDS is sub

Externí odkaz: https://doaj.org/article/b0fbe708443d4fff8cc1f3c1466bfdbb

Systematic assessment of variability in the proteome of iPSC derivatives

Autor: Stephanie D. Beekhuis-Hoekstra, Kyoko Watanabe, Josefin Werme, Christiaan A. de Leeuw, Iryna Paliukhovich, Ka Wan Li, Frank Koopmans, August B. Smit, Danielle Posthuma, Vivi M. Heine

Publikováno v: Stem Cell Research, Vol 56, Iss , Pp 102512- (2021)

The use of induced pluripotent stem cells (iPSC) to model human complex diseases is gaining popularity as it allows investigation of human cells that are otherwise sparsely available. However, due to its laborious and cost intensive nature, iPSC rese

Externí odkaz: https://doaj.org/article/17cd00d645064ceab99c936775f4a01d

Blue Native PAGE–Antibody Shift in Conjunction with Mass Spectrometry to Reveal Protein Subcomplexes

Autor: Miao Chen, Frank Koopmans, Iryna Paliukhovich, Sophie J. F. van der Spek, Jian Dong, August B. Smit, Ka Wan Li

Publikováno v: International Journal of Molecular Sciences, 24(8):7632, 1-14. Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences; Volume 24; Issue 8; Pages: 7632
Chen, M, Koopmans, F, Paliukhovich, I, van der Spek, S J F, Dong, J, Smit, A B & Li, K W 2023, ' Blue Native PAGE–Antibody Shift in Conjunction with Mass Spectrometry to Reveal Protein Subcomplexes : Detection of a Cerebellar α1/α6-Subunits Containing γ-Aminobutyric Acid Type A Receptor Subtype ', International Journal of Molecular Sciences, vol. 24, no. 8, 7632, pp. 1-14 . https://doi.org/10.3390/ijms24087632

The pentameric γ-Aminobutyric acid type A receptors (GABAARs) are ligand-gated ion channels that mediate the majority of inhibitory neurotransmission in the brain. In the cerebellum, the two main receptor subtypes are the 2α1/2β/γ and 2α6/2β/δ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::729d1b2a87dfbdad83a37e28de13e056
https://research.vu.nl/en/publications/2a1fa5a0-e958-418e-ba0d-7902a5b01175